精子蛋白ERp57与宫腔内人工授精周期妊娠率相关性的初步分析

目的:比较不同周期妊娠率的宫腔内人工授精(IUI)所用的精子中,精子蛋白ERp57表达量的差异,探讨其能否作为筛选合格供精者精液的一项指标。方法:将42例用于IUI的精液标本,按IUI周期妊娠率分为A组(16例,IUI周期妊娠率为0)、B组(13例,IUI周期妊娠率为10%～20%)和C组(13例,IUI周期妊娠率大于20%),应用Western印迹检测精子蛋白ERp57的表达情况,其表达量用ERp57蛋白与内参β-微管蛋白的平均吸光度的比值表示。结果:A、B、C组精子蛋白ERp57的表达量分别为:0.95±0.24、1.33±0.43和1.33±0.39,ERp57蛋白在A组中的表达量明显低于B组与C组(P<0.05),而B组与C组之间则无明显差异(P>0.05)。结论:ERp57蛋白在A组(IUI周期妊娠率为0)精子中的低表达,可以作为筛选不合格供精者及精子库中低IUI周期妊娠率的精液标本原因分析的一项参考指标。

Correlation Between ERp57 and Pregnancy of Intrauterine Insemination

Objective： To investigate the association between the pregnancy rate of intrauterine insemination （IUI） and the ERp57 expression level in donor sperm. Methods： Forty-two sperm samples were divided into 3 groups according to the pregnancy rate： Group A （n = 16, pregnancy rate = 0）, B （n = 13, pregnancy rate 10%-20% ） and C （n = 13, pregnancy rate ≥ 20% ）. The optical density （OD） was normalized to the β-tubulin band for the evaluation of the ERp57 level. Results： OD （ERp57/Tubulin） was 0. 95 ± 0. 24 in Group A, 1.33 ± 0. 43 in Group B and 1.33 ± 0.39 in Group C. The ERp57 expression level was significantly lower in Group A than in B and C （P 〈 0. 05）, with no significant differences between the latter two groups. Conclusion ： The ERp57 expression level in donor sperm could be used as an index to predict the pregnancy rate of IUI and to avoid IUI failure by removing lowlevel donor sperm.