| 丹参分支酸变位酶基因的克隆和生物信息学分析 |
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| 引用本文： | 王亚君,黄璐琦,蒋超,申业. 丹参分支酸变位酶基因的克隆和生物信息学分析[J]. 中国中药杂志, 2013, 38(11): 1697-1702 |
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| 作者姓名： | 王亚君 黄璐琦 蒋超 申业 |
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| 作者单位： | 中国中医科学院中药资源中心,北京,100700 |
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| 基金项目： | 国家自然科学基金项目(81173490,81130070) |
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| 摘 要： | ![]()
应用BlastX检索丹参毛状根的EST数据库,发现1条与分支酸变位酶(chorismate mutase,CM)高度同源的序列,克隆得到其全长cDNA,命名为SmCM1,Genbank登录号为KC784342.SmCM1全长948 bp,理论pI 6.41,与矮牵牛Petunia×hybriida、拟南芥Arabidopsis thaliana和毛果杨Populus trichocarpa的CM1分别具有70％,72％,64％的相似性.实时荧光定量PCR(real time quantitative PCR,QRT-PCR)分析表明SmCM1在丹参叶中的含量较高,其次是茎,在根中的含量相对较低.酵母诱导子(YE)和离子(Ag+)联合诱导丹参毛状根后,SmCM1与莽草酸途径的关键酶3-脱氧-D-阿拉伯-庚酮糖-7-磷酸合酶(3-deoxy-7-phosphoheptulonate synthase,DAHPS)和分支酸合酶(chorismate synthase,CS)基因表达趋势呈协同变化.YE+ Ag+处理后3个基因皆上调表达,8h达到表达高峰,分别是对照的7.9,5.5,9.8倍,随后下调,36 h时下降至对照水平以下,说明诱导处理之后糖代谢中间产物经莽草酸和分支酸途径合成苯丙氨酸和酪氨酸从而引起酚酸类化合物的过量积累.
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| 关 键 词： | 丹参 分支酸变位酶 基因克隆 丹参毛状根 生物信息学 |
| 收稿时间： | 2013-02-06 |
Cloning and bioinformatics analysis of chorismate mutase gene from Salvia miltiorrhiza |
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| WANG Ya-jun,HUANG Lu-qi,JIANG Chao and SHEN Ye. Cloning and bioinformatics analysis of chorismate mutase gene from Salvia miltiorrhiza[J]. China Journal of Chinese Materia Medica, 2013, 38(11): 1697-1702 |
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| Authors: | WANG Ya-jun HUANG Lu-qi JIANG Chao SHEN Ye |
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| Affiliation: | National Resource Center for Chinese Materia Medica, China Academy of Chinese medical sciences, Beijing 100700, China;National Resource Center for Chinese Materia Medica, China Academy of Chinese medical sciences, Beijing 100700, China;National Resource Center for Chinese Materia Medica, China Academy of Chinese medical sciences, Beijing 100700, China;National Resource Center for Chinese Materia Medica, China Academy of Chinese medical sciences, Beijing 100700, China |
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| Abstract: | ![]()
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| Keywords: | Salvia miltiorrhiza chorismate mutase bioinformatics analysis |
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