茉莉酸甲酯对曼陀罗毛状根中主要莨菪烷类生物碱成分积累和释放的影响

目的:研究茉莉酸甲酯(methyl jasmonate,MJ)对曼陀罗毛状根中主要次生代谢产物东莨菪碱和莨菪碱成分的积累和向培养基中的释放的影响.方法:1/2 MS液体培养C58C1农杆菌诱导的曼陀罗毛状根培养18 d后,液体培养基中加入化学诱导子——茉莉酸甲酯,采用HPLC测定MJ处理后不同浓度梯度及不同时间段(0,3,6,9,12 d)曼陀罗毛状根中东莨菪碱和莨菪碱的含量以及其在培养基中的含量.结果:曼陀罗毛状根经MJ处理后3,6,9,12 d的东莨菪碱的质量分数分别达0.419,0.439,0.431,0.374 mg·g-1,分别是同时期未经MJ处理的1.36,1.42,1.17,1.12倍；莨菪碱的质量分数达1.493,0.817,0.723,0.698 mg·g-1,分别是同时期未经MJ处理曼陀罗毛状根的2.28,1.11,0.63,0.70倍.结论:MJ能显著促进曼陀罗毛状根中东莨菪碱成分的积累并向培养基中释放；MJ能显著促进处理3d和6d后曼陀罗毛状根中莨菪碱成分的积累并向培养基中释放.

Effects of methyl jasmonate on accumulation and release of main tropane alkaloids in liquid cultures of Datura stramonium hairy root

Objective: To study the effects of methyl jasmonate (MJ) on the accumulation and release of main secondary metabolites i.e. scopolamine and hyoscyamine in liquid cultures of Datura stramonium hairy roots. Method: After 18 days liquid culture of D. stramonium hairy roots induced by agrobacterium rhizogenes C58C1, the chemical elicitor methyl jasmonate was added into 1/2 MS liquid cultures and scopolamine and hyoscyamine on the day 0, 3, 6, 9 and 12, after dealing with MJ, was determined by HPLC. Result: After dealing with MJ on the day 3, 6, 9 and 12,the concentration of scopolamine reached to 0.419,0.439,0.431,0.374 mg·g^-1, respectively, the increase of scopolamine were 1.36,1.42,1.17 and 1.12 fold higher than that of the control, respectively. And hyoscyamine reached 1.493,0.817,0.723 and 0.698 mg·g^-1, respectively, the increase of hyoscyamine were 2.28,1.11,0.63 and 0.70 fold higher than that of the control, respectively. Conclusion: MJ could stimulate the accumulation of scopolamine and hyoscyamine(3,6 d) in D. stramonium hairy root and have released them into the culture medium.