|
|||||
|
|
Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia |
|
| Authors: | Keiko Yamanaka Nikolaos V Sofikitis Ikuo Miyagawa Yasuhisa Yamamoto Toshiko Toda Spyros Antypas Dimitrios Dimitriadis Manami Takenaka Koji Taniguchi Katsuhiko Takahashi Sanae Tsukamoto Hiroshi Kawamura Martin Neil |
| Institution: | (1) Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan;(2) Japanese-Greek Fertility Institute, Athens, Greece;(3) Infertility Clinic, Matsue Seikyo Hospital, Matsue, Japan;(4) Department of Surgery, Agia Sofia Hospital, Andrology Unit, Athens, Greece;(5) Hiroshima HART Clinic, Hiroshima, Japan |
| Abstract: | Purpose: Our objective was to apply ooplasmic round spermatid nuclear injections for the treatment of nonobstructive azoospermia. Materials: Participants were nine azoospermic men who had previously undergone diagnostic testicular biopsy. Spermatogenetic arrest was diagnosed at the round spermatid stage (n=6) or primary spermatocyte stage (n=3). A second (therapeutic) testicular biopsy was performed and round spermatid nuclei were recovered from all the participants. Results: Forty-nine mature oocytes were successfully injected with nuclei and then cultured for 72 hr. Twenty-four embryos were transferred to nine women. No pregnancy was achieved. Conclusions: Round spermatids can be recovered from therapeutic testicular biopsy material of men negative for round spermatids in previous routine diagnostic testicular biopsy specimens. Round spermatid nuclear injections may play a role in the treatment of nonobstructive azoospermia. |
| Keywords: | azoospermia infertility oocyte round spermatid |
| 本文献已被 SpringerLink 等数据库收录！ | |