近红外光谱法快速测定酒女贞子中女贞苷含量

目的:利用近红外光谱技术(NIRS)快速测定酒女贞子中女贞苷含量。方法:采用3个不同产地的黄酒对女贞子进行炮制,以HPLC测定样品中女贞苷的含量为参考,运用近红外光谱技术结合偏最小二乘法(PLS)建立酒女贞子中女贞苷含量的定量分析模型。结果:女贞苷的定量分析模型内部交叉验证决定系数(R2)为0.967 89,校正均方根偏差(RMSEC)为0.107,预测均方根偏差(RMSEP)为0.103,交互验证均方根偏差(RMSECV)为0.256 69,主因子数为8。结论:该方法简便快速,准确无损,所建模型能够对酒女贞子中女贞苷的含量进行快速测定。

Fast Determination of Ligustroflavone in Wine Ligustri Lucidum Fructus by Near Infrared Spectroscopy

Objective: To determine the content of ligustroflavone in wine Ligustri Lucidum Fructus by near infrared spectroscopy. Method: Ligustri Lucidum Fructus was processed with ricewine from three different regions. With the content of ligustroflavone determined by HPLC as reference value, quantitative model of ligustroflavone content in wine Ligustri Lucidum Fructus was established by using near infrared spectroscopy combined with partial least squares (PLS). Result: The correlation coefficients (R²) of the quantitative calibration model for ligustroflavone was 0.96789;root mean square error of calibration (RMSEC) was 0.107;root mean square error of predication (RMSEP) was 0.103;root mean square error of cross validation (RMSECV) was 0.25669, and the number of main factors was 16. Conclusion: This method is convenient and accurate. The established models can realize fast determination of ligustroflavone in wine Ligustri Lucidum Fructus.