SLE患者外周血中Foxp3+CD4+CD25+调节性T细胞的分析

目的分析SLE患者外周血中Foxp3 CD4 CD25 调节性T细胞和T细胞亚群上GITR的表达,以初步阐述其在SLE患者免疫稳态调节中的作用和意义。方法以流式细胞术检测SLE患者外周血中Foxp3 CD4 CD25 调节性T细胞和T细胞亚群上GITR的表达。结果稳定期及活动期SLE患者外周血中Foxp3 CD4 CD25 调节性T细胞比例显著低于健康对照(P<0.05),且活动期SLE患者Foxp3 CD4 CD25 调节性T细胞比例高于稳定期SLE患者(P>0.05)。SLE患者外周血CD3 CD4 T细胞和CD3 CD8 T细胞上GITR表达显著增加(P<0.05);随着疾病活动性增加,CD3 CD4 T细胞上GITR表达降低(P>0.05),CD3 CD8 T细胞上GITR表达增加(P>0.05)。结论SLE患者外周血中Foxp3 CD4 CD25 调节性T细胞表达降低,而患者T细胞亚群上GITR表达增加,其共同作用在诱导SLE患者外周耐受障碍中具有重要意义。

Analysis of Foxp3+CD4+CD25+ regulatory cells in peripheral blood of patients with SLE

AIM: To investigate the expression of GITR on T cells and the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood of patients with SLE. METHODS: The expression of GITR and Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood was determined by flow cytometry(FCM). RESULTS: Compared with health control, the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in peripheral blood from SLE patients was lower (P<0.05), but there is no difference in the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells between active and inactive SLE patients (P>0.05). GITR expression on both CD3(+) CD4(+) T cells and CD3(+) CD8(+) T cells of SLE patients increased significantly (P>0.05). With the development of SLE activity, GITR expression didn't change significantly on both CD3(+) CD4(+) T cells (P>0.05) and CD3(+) CD8(+) T cells (P>0.05). CONCLUSION: The expression abnormality of both Foxp3(+) CD4(+) CD25(+) regulatory cells and GITR may play important role in the imbalance of immune homeostasis in SLE.