In vivo release of substance P in cat dorsal horn studied with microdialysis |
| |
| Authors: | E Brodin B Linderoth B Gazelius U Ungerstedt |
| |
| Affiliation: | 1. Department of Pharmacology, Karolinska Institutet, Stockholm Sweden;2. Department of Neurosurgery, Karolinska Hospital, Stockholm Sweden;3. Department of Endodontics, Karolinska Institutet, Stockholm Sweden;1. Université Paris-Descartes, Paris, France;2. Service de Médecine Interne, Centre de Référence Maladies Auto-Immunes et Systémiques Rares, Hôpital Cochin, AP-HP, Paris, France;1. Department of Pharmacology, Hebei Medical University, Shijiazhuang, 050011, PR China;2. Faculty of Medicine and Health, University of Leeds, Leeds, UK;3. School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds, UK;1. Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London WC1E 6BT, UK;2. Department of Psychiatry, University of California San Diego, La Jolla, CA, USA;3. Research Service, VA San Diego Healthcare System, San Diego, CA, USA;1. Veteran Affairs Greater Los Angeles Healthcare System, 11310 Wilshire Blvd., Building 115, Los Angeles, CA 90073, USA;2. Vatche and Tamar Manoukian Division of Digestive Diseases, 900 Veterans Ave., Warren Hall Building, Department of Medicine, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA;1. Department of Neurobiology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA;2. Department of Otolaryngology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA;3. Pittsburgh Center for Pain Research, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15213, USA |
| |
| Abstract: | Variations in the extracellular concentration of substance P (SP) were measured in cat dorsal horn in vivo by microdialysis and radioimmunoassay. Electrical unilateral stimulation of the sciatic nerve was used to evoke release of SP. At high-intensity stimulation, activating slowly conducting (approx. 0.9 m/s) fibres, there was an increase in substance P-like immunoreactivity (SP-LI) to 338% of the preceding control value (P less than 0.05, n = 5) in the dialysates collected at levels L6-L7 at the stimulated side. A less pronounced increase, to 164% of the basal level, was found at the contralateral side. More cranially (L1-L5) in the dorsal horn at the stimulated side, only a moderate or no increase in SP-LI was seen. Stimulation with low intensity, sufficient to activate fast (greater than 20 m/s), but not slowly conducting fibres, evoked no increase in SP-LI. The results indicate that unilateral peripheral C-fibre activation induces a local SP release in the dorsal horn present also on the side not stimulated. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
