| Detection of the copy number of plasmid encoding HBsAg in recombinant yeast by PCR relative quantitative assay |
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| 引用本文： | YUN HONG~1,JIN LI~2,HE MU WANG~3 and KAI ZHAO~1~1 National Vaccine & Serum Institute,Beijing,P. R. China~2 Beijing Tiantan Biological Products Co.,Ltd,Beijing,P. R. China~3 Tianjin Bohui Biotechnology Company,Tianjin,P. R. China. Detection of the copy number of plasmid encoding HBsAg in recombinant yeast by PCR relative quantitative assay[J]. 中华微生物学和免疫学杂志(英文版), 2005, 3(4): 274-279 |
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| 作者姓名： | YUN HONG~1 JIN LI~2 HE MU WANG~3 and KAI ZHAO~1~1 National Vaccine & Serum Institute Beijing P. R. China~2 Beijing Tiantan Biological Products Co. Ltd Beijing P. R. China~3 Tianjin Bohui Biotechnology Company Tianjin P. R. China |
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| 作者单位： | YUN HONG~1，JIN LI~2，HE MU WANG~3 and KAI ZHAO~1~1 National Vaccine & Serum Institute，Beijing，P. R. China~2 Beijing Tiantan Biological Products Co.，Ltd，Beijing，P. R. China~3 Tianjin Bohui Biotechnology Company，Tianjin，P. R. China |
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| 摘 要： | Thegeneticrecombinantyeast,Saccharomyces cerevisiae,carryingtheplasmidscontainingthe hepatitisBvirussurfaceantigen(HBsAg)encod ingregionisemployedtoproducetherecombinant hepatitisBvaccine.Someplasmidsoftherecom binantyeastmaybelostduringindustrialferment ationtherebyinfluencingtheexpressionofHB sAg.Eitheratthepracticalfermentationtoex pressHBsAgformakingvaccineorrenovationoffermentationprocessoftherecombinantyeastto enhancetheyieldsofHBsAg,suchasextension ofculturingperiod,theplasmidstat…
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| 关 键 词： | 编码技术 HBsAg 重组细胞 酵母 |
Detection of the copy number of plasmid encoding HBsAg in recombinant yeast by PCR relative quantitative assay |
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| YUN HONG JIN LI HE MU WANG KAI ZHAO. Detection of the copy number of plasmid encoding HBsAg in recombinant yeast by PCR relative quantitative assay[J]. Chinese Journal of Microbiology and Immunology, 2005, 3(4): 274-279 |
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| Authors: | YUN HONG JIN LI HE MU WANG KAI ZHAO |
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| Abstract: | |
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| Keywords: | Recombinant Saccharomyces cerevisiae Plasmid copies Real-time PCR Relative quantification |
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