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| IL-6抑制LPS诱导的小鼠髓源性DCs成熟的实验研究 | |
| 引用本文： | 赵永亮,余佩武,罗华星,雷晓,刘伟,饶芸. IL-6抑制LPS诱导的小鼠髓源性DCs成熟的实验研究[J]. 重庆医学, 2010, 39(8) |
| 作者姓名： | 赵永亮 余佩武 罗华星 雷晓 刘伟 饶芸 |
| 作者单位： | 第三军医大学西南医院普外科,重庆,400038;第三军医大学西南医院普外科,重庆,400038;第三军医大学西南医院普外科,重庆,400038;第三军医大学西南医院普外科,重庆,400038;第三军医大学西南医院普外科,重庆,400038;第三军医大学西南医院普外科,重庆,400038 |
| 摘 要： | 目的观察白细胞介素-6(IL-6)对于内毒素(LPS)诱导的小鼠髓源性树突状细胞(DC)成熟效应的抑制作用。方法分离小鼠骨髓细胞,采用黏附法结合GM-CSF和IL-4刺激法在体外培养髓源性DCs(BMDCs)。用相差倒置显微镜观察BM-DCs的形态特点。用LPS诱导DC成熟。采用FACS结合混合淋巴细胞反应,观察IL-6处理与否,LPS诱导的BMDCs在表型和刺激同种淋巴细胞增殖功能上的变化。结果形态观察和FACS分析的结果表明:体外成功培养了纯度较高的高表达CD11c的BMDCs,LPS能明显诱导BMDCs表面MHC-Ⅱ类分子和共刺激分子CD40的表达上调;而经过IL-6处理后,LPS诱导BMDCs表面CD80、CD86、CD40表达上调的能力被显著削弱(P0.05)。混合淋巴细胞反应显示,与单独用LPS处理的BMDCs相比,经IL-6处理后的BMDCs刺激同种淋巴细胞增殖的能力明显减弱,并呈剂量依赖关系(P0.05)。结论IL-6能明显抑制LPS诱导的BMDCs成熟,可能是肿瘤免疫逃逸的重要机制之一。 |
| 关 键 词： | 髓源性树突状细胞 白细胞介素-6 脂多糖 |
Suppression effect of IL-6 on the maturation of mouse bone marrow derived dendritic cells induced by LPS |
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| ZHAO Yong-liang,YU Pei-wu,LUO Hua-xing,et al.. Suppression effect of IL-6 on the maturation of mouse bone marrow derived dendritic cells induced by LPS[J]. Chongqing Medical Journal, 2010, 39(8) | |
| Authors: | ZHAO Yong-liang YU Pei-wu LUO Hua-xing et al. |
| Affiliation: | ZHAO Yong-liang,YU Pei-wu,LUO Hua-xing,et al.(Department of General Surgery,Southwest Hospital,The Third Military Medical University,Chongqing 400038,China) |
| Abstract: | Objective To explore the suppression effect of IL-6 on the maturation of mouse bone marrow-derived dendritic cells(BMDCs) induced by LPS.Methods To obtain immature BMDCs,mouse bone marrow cells were isolated and cultured in complete culture medium containing GM-CSF and IL-4 following the removement of the nonadherent cells.The cell morphology was observed by using a phase contrast inverted microscope.The maturation of BMDCs was induced by LPS.The expression of surface costimulation molecules on LPS-treated ... |
| Keywords: | bone marrow derived dendritic cells(BMDCs) interleukin(IL-6) lipopolysaccharide(LPS) |
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