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| 重组人铜锌SOD的部分理化性质研究 | |
| 引用本文： | 高俊杰,夏文超,袁勤生. 重组人铜锌SOD的部分理化性质研究[J]. 药物生物技术, 2003, 10(1): 43-47 |
| 作者姓名： | 高俊杰 夏文超 袁勤生 |
| 作者单位： | 华东理工大学生物反应器工程国家重实验室,上海,200237 |
| 摘 要： | 对rhCu，Zn-SOD进行SDS-PAGE测定该SOD的亚基分子质量为19ku，该酶对热稳定，在pH5左右的活力最高，对乙腈、EDTA等抑制剂敏感，但是对SDS、尿素等变性剂表现出很好的稳定性。对该酶进行光谱分析表明，在265nm和673nm具有吸收峰，原子吸收光谱分析表明每分子SOD含有2个铜原子和2个锌原子，ESR表明铜原子价态为2价。可认为该重组酶与天然Cu，Zn-SOD在理化性质和结构上基本相同。 |
| 关 键 词： | 重组人铜锌SOD 理化性质 铜锌超氧化物歧化酶 |
| 文章编号： | 1005-8915(2003)01-0043-05 |
Study on the Properties of Recombinant Human Cu, Zn-SOD |
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| GAO Jun-jie,XIA Wen-chao,YUAN Qin-sheng. Study on the Properties of Recombinant Human Cu, Zn-SOD[J]. Pharmaceutical Biotechnology, 2003, 10(1): 43-47 | |
| Authors: | GAO Jun-jie XIA Wen-chao YUAN Qin-sheng |
| Abstract: | The molecular weihgt of the subunit of rhCu, Zn-SOD is 19 ku, determined by SDS-PAGE, Thee recombi-nant enzyme shows high thennostability and pH stability. It is also stable when treated with such as SDS and urea but sensitive to acetonitrile and EDTA. The enzyme shows two absorption peak (265nm and 673nm, respectively) in the ultraviolet and visible light area. The atomic absorption spectrum indicates that each enzyme molecular ahve two Cu atoms and two Zn atoms. ESR spectrum indicates that the valence of the copper atom is 2. |
| Keywords: | Recombinant human Cu Zn-SOD Properties |
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