| 紫外线与乙醇协同对枯草杆菌黑色变种芽胞杀灭机理的初步研究 |
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| 引用本文： | 刘怀田,李荣芬.紫外线与乙醇协同对枯草杆菌黑色变种芽胞杀灭机理的初步研究[J].中国消毒学杂志,1994,11(4):197-205. |
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| 作者姓名： | 刘怀田 李荣芬 |
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| 作者单位： | 北京军事医学科学院微生物流行病研究所 |
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| 摘 要： | 试验表明，紫外线与乙醇协同作用于枯草杆菌黑色变种芽胞，较单用紫外线照射，可使壳层肿胀，核心电子密度下降，吡啶二羧酸漏出量增加。乙醇经紫外线光解可产生较多的自由基ＯＨ，该自由基可能对杀芽胞的协同作用有一定意义。
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| 关 键 词： | 紫外线，乙醇，协同杀菌机理，枯草杆菌黑色变种芽胞，吡啶二羧酸，自由基 |
PRELIMINARY STUDY ON MECHANISM OF SYNERGETIC KILLING EFFECT OF ULTRAVIOLET
RAYS AND ETHANOL ON SPORES OF Bacillus subtilits var.niger |
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| Liu Huaitian,LiRongfen.PRELIMINARY STUDY ON MECHANISM OF SYNERGETIC KILLING EFFECT OF ULTRAVIOLET
RAYS AND ETHANOL ON SPORES OF Bacillus subtilits var.niger[J].Chinese Journal of Disinfection,1994,11(4):197-205. |
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| Authors: | Liu Huaitian LiRongfen |
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| Abstract: | The synergetic killing effect of
ultraviolet rays and ethanol on spores of Bacillus subtilisvar.niger,the changes of dipicolinic
acid content and free radical production were studied inthe present experiment.The
ultrastructural changes of the spore were also observed underelectronmicroscope. The results
indicated that 99.99% of the spores of Bacillus subtilis var.niger could be kil-led after exposure
to synergetic action of ultraviolet rays and ethanol for 5 min,wMileultraviolet irradiation only
killed 95.42 % and 75%ethanol had no killing effect,As comparedwith the ultraviolet-irradiated
spores,the sporesexpo sed to the synerge tic action ofultraviolet rays and ethanol showed
obvious swelling of the coat,decrease in electron densityof the core,increased leakage of
dipicolinic acid and increased production of free radicalOH。The free radica.l OH may play a
certain role in the synergetic killing effect of thesetwo factors on the spores of Bacillus subtilis
var.niger. |
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| Keywords: | ultravioletrays ethanol mechanism of synergetic germicidal action Bacillus subtilis var niger spore dipicolinic acid free radical |
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