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| HPLC法测定苦参碱氯化钠注射液中苦参碱含量 | |
| 引用本文： | 韩春英,王津津,许华英. HPLC法测定苦参碱氯化钠注射液中苦参碱含量[J]. 齐鲁药事, 2008, 27(3): 151-152 |
| 作者姓名： | 韩春英 王津津 许华英 |
| 作者单位： | 1. 德州德药常药有限公司,山东,德州,253015 2. 德州市立医院,山东,德州,253012 |
| 摘 要： | 目的建立HPLC法测定苦参碱氯化钠注射液中苦参碱的含量。方法用C18色谱柱(250mm×4.6mm,5μm),以0.025mol.L-1磷酸溶液(用三乙胺调节pH值至3.0)-乙腈(95∶5)为流动相,流速1mL.min-1,检测波长220nm。结果苦参碱浓度在40~120μg.mL-1范围内呈良好的线性关系,r=0.9998。平均回收率为99.81%,RSD为0.55%(n=9)。结论本法操作简便、结果准确可靠,可用于苦参碱氯化钠注射液质量控制。 |
| 关 键 词： | 苦参碱氯化钠注射液 苦参碱 含量测定 高效液相色谱法 |
| 文章编号： | 1672-7738(2008)03-0151-02 |
Determination of matrine in Matrine Sodium Chloride Injection by HPLC |
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| HAN Chun-ying,WANG Jin-jin,XU Hua-ying. Determination of matrine in Matrine Sodium Chloride Injection by HPLC[J]. qilu pharmaceutical affairs, 2008, 27(3): 151-152 | |
| Authors: | HAN Chun-ying WANG Jin-jin XU Hua-ying |
| Abstract: | OBJECTIVE To establish an HPLC method for determination of matrine in Matrine Sodium Chloride Injection.METHODS C18(250mm×4.6mm,5μm) column was used.Mobile phase of 0.025mol·L-1 phosphoric acid(adjusted to pH3.0 with triethylamine)-acetonitrile(95∶5) with flow rate 1mL·min-1,detection wavelength was 220nm.RESULTS The calibration curve was linear in the range of 40~120μg·mL-1,r=0.9998.The average recovery of matrine was 99.81% and RSD was 0.55%(n=9).CONCLUSION This method was simple and convenient in the operation.It was also proposed to be used for the quality control of Matrine Sodium Chloride Injection. |
| Keywords: | Matrine Sodium Chloride Injection matrine determination HPLC |
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