抗环瓜氨酸肽抗体与类风湿因子联合检测在类风湿关节炎诊断中的意义

目的探讨联合检测抗环瓜氨酸肽（CCP）抗体和类风湿因子（RF）在类风湿关节炎（RA）诊断中的意义。方法抗-CCP采用ELISA法,RF采用散射速率比浊法检测,检测59例RA、48例非RA患者血清抗抗-CCP和RF。结果59例RA患者中单一抗-CCP阳性8例,单一RF阳性16例,两项同时阳性30例,同时阴性5例,故抗-CCP、RF及两者联合检测在RA患者中的阳性率分别为64.40%、77.97%和91.53%;48例非RA患者中单一抗-CCP阳性2例,单一RF阳性14例,无1例两项同时阳性,故抗-CCP、RF及抗-CCP和RF联合检测在非RA患者中的阳性率分别为4.16%、29.16%和0。经过统计学分析联合检测与单项检测比较差异有统计学意义（P＜0.01）,且RA患者抗-CCP与RF阳性率显著高于非RA患者,差异有统计学意义（χ2=41.15、25.60,P＜0.01）。结论抗-CCP和RF联合检测可明显提高RA的诊断率。

Diagnostic significance of combined detection of serum anti cyclic citrullinated-peptide antibody and rheumatoid factor in rheumatoid arthritis

Objective To evaluate the significance of the combination of serum anti cyclic citrullinated peptide antibody （anti CCP） and rheumatoid factor （RF） in the diagnosis of rheumatoid arthritis （RA）. Methods ELISA and IRN techniques were used to detect serum levels of anti CCP and RF in 59 patients with RA and 48 patients without RA. Results Positive anti CCP or RF alone was found in 8 or 16 patients with RA,respectively. while positive anti CCP and RF were found in 30 patients with RA. Positive anti CCP or RF alone was only noticed in two or 14 patients without RA. Both anti-CCP and RF positive were not observed in the patients without RA. The positive rate of anti CCP or RF alone and both of patients with RA was 64.40%, 77.97 %, and 91.53 % respectively. While the positive rate of anti CCP or RF alone and both of patients without RA was 4. 16%, 29. 16% ,and 0 respectively. Through the analysis of statistics,the combined examination as well as the single examination great significance statistically. The positive rate of anti-CCP and RF of patients with RA was significantly higher than that of non-RA patients（X^2 = 41.15,25.60 ;P〈0.01）. Conclusion Determination of the combination of serum anti CCP and RF is helpful in the diagnosis of patients with RA.