SOX10在毛细胞型星形细胞瘤和室管膜肿瘤鉴别诊断中的应用

目的检测SOX10在毛细胞型星形细胞瘤(pilocytic astrocytoma,PA)/毛细胞黏液样星形细胞瘤(pilomyxoid astrocytoma,PMA)及室管膜肿瘤中的表达,并比较在不同WHO分级、年龄组及解剖部位的PA/PMA及室管膜肿瘤中SOX10的表达差异。方法采用免疫组化EnVision两步法检测52例PA,9例PMA及205例室管膜肿瘤中SOX10的表达。结果PA/PMA中,SOX10的阳性率为100%,且均为强阳性,在不同年龄组及解剖部位SOX10的表达差异无显著性(P均>0.05);室管膜肿瘤中,仅6%(12/205)SOX10呈阳性,不同WHO分级、年龄组及解剖部位的室管膜肿瘤中SOX10表达差异无显著性(P均>0.05)。SOX10在PA/PMA与室管膜肿瘤中的阳性率差异有显著性(P<0.05)。结论SOX10选择性高表达于PA/PMA,对其诊断及与室管膜肿瘤的鉴别诊断具有重要意义。

Application of SOX10 in differential diagnosis between pilocytic astrocytoma and ependymal tumours

Purpose To study SOX10 expression in pilocytic astrocytoma (PA), pilomyxoid astrocytoma (PMA) and ependymal tumours, and to compare its expression difference in PA/PMA and ependymal tumours with different grade, age and anatomic site. Method Immunohistochemical EnVision method was uesed to detect the expression of SOX10 in 52 cases of PA, 9 cases of PMA and 205 cases of ependymal tumours. Result In PA/PMA, the positive rate of SOX10 expression was 100%, and all of them were strongly positive. There was no significant difference in the expression of SOX10 among different age and anatomic site( P >0.05). In ependymal tumours, only 6%(12/205) of ependymal tumours were immunopositive for SOX10. No significant difference in SOX10 expression was observed within groups of different grade, age and anatomic site( P >0.05). The percentage of SOX10 expression showed significant difference between PA/PMA and ependymal tumours ( P <0.05). Conclusion Selective high expression of SOX10 in PA/PMA may be useful for the diagnosis and differential diagnosis of PA/PMA.