sCXCL16对弥漫大B细胞淋巴瘤的体外生物学影响与初步机制

目的探讨s CXCL16对弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)细胞体外生物学特征的影响,并分析其初步机制。方法采用CCK8法观察DLBCL细胞株增殖能力;应用Transwell法检测细胞的迁徙能力,全基因组表达谱芯片筛选不同干预组差异表达的基因;运用Western blot检测差异基因的蛋白表达。结果外源性s CXCL16重组蛋白对DLBCL细胞增殖影响差异无显著性,但可促进DLBCL细胞自身的迁移并具有剂量依赖性,使用CXCR6抗体可阻断该作用;外源性添加重组蛋白s CXCL16可影响DLBCL细胞多种基因表达,其中趋化因子配体/受体途径相关的13种基因出现上调; Western blot结果证实外源性添加s CXCL16重组蛋白可上调TNFRSF12A蛋白表达,而使用CXCR6抗体则降低其表达。结论 s CXCL16可能通过其自身受体CXCR6,以剂量依赖性模式促进DLBCL细胞的迁移,并调控多种趋化因子配体/受体的表达。

Effect of soluble CXCL16 on diffuse large B cell lymphoma in vitro and its preliminary mechanism

Purpose This study was to examine the effect of sCXCL16 on the biological characteristics of diffuse large B cell lymphoma (DLBCL) cells in vitro and to explore its preliminary mechanism. Methods The proliferation and migration ability of DLBCL cell were investigated using CCK-8 and Transwell assay,respectively. The differentially expressed genes in different intervention groups were screened using genome-wide expression microarray,and some of the key proteins were detected using Western blot. Results The effect of exogenous recombinant sCXCL16 on DLBCL cell proliferation was not significant, while the DLBCL cell migration was found to be promoted in a dose-dependent manner,which could be blocked by anti-CXCL16 or anti-CXCR6 antibody. Results by using genome-wide expression microarray showed that various genes were affected including 13 kinds of chemokine ligands/receptors,among which tumor necrosis factor (TNF) receptor SF12A (TNFRSF12A) protein was confirmed to be up-regulated by Western blot assay. Conclusion In DLBCL cells,sCXCL16 may promote the migration of DLBCL cells through its unique receptor-CXCR6 in a dose-dependent manner and regulate the expression of various chemokine ligands/receptors.