锶磷灰石修复颌骨缺损的实验研究

目的以不同含锶量的锶磷灰石(Sr-HAP)植入动物体内,进行各项生物学测试,为该材料的临床实际应用作前期研究.方法将24只新西兰大白兔分为3组,双侧下颌角均造成约6mm×12mm×4mm的缺损,用10%、5%、0%的Sr-HAP分别予以修复,于1、3、6个月时随机处死一组分别进行尸解、光镜、扫描电镜、四环素荧光标记、定量组织学观察.结果锶磷灰石人工骨植入后无感染和排斥反应,术后材料的部分降解早,有部分诱导成骨现象,5%和10%的锶磷灰石成骨量与纯羟基磷灰石(0%)比较在统计学上有差异,而5%与10%的锶磷灰石成骨量统计学上差异无显著性.结论①锶磷灰石有良好的组织相容性、骨引导性及生物降解性,并可能具有一定程度的骨诱导性,锶的存在提高了新骨的总生成量,延长了新骨生成的总体时间和高峰期.②锶磷灰石具有良好的生物降解率和合适的生物降解速率,能获得较满意的骨缺损修复效果.

The experimental study of Sr-HAP on reconstructing mandibular bone defect

OBJECTIVE: Different ration of Sr-HAP was implanted in animals to study the bioreactions in order to prepare for the clinical applications in future. METHODS: 24 rabbits were divided into 3 groups for study. Bone defect of 6 mm x 12 mm x 4 mm was made at the mandibular angle of rabbits and Sr-HAP of different proportion (10%, 5%, 0%) was applied to reform the defect. One group of animals were killed at 1 month, 3 months, 6 months to evaluate the biologic capacity with anatomy, histology, SEM, tetracycline fluorescent marker, ECT and ration histology. RESULTS: Sr-HAP had hardly been rejected by hosts, and in early period after operation the new bone in the Sr-HAP was found with great quantities as the degradation of Sr-HAP much more than that in the pure HA, and the difference of bone quantity can be shown in statistics. CONCLUSIONS: 1. Sr-HAP has a better biocompatibility, biodegradation than pure HA and a excellent osteoinductivity. The existence of Sr-HAP improves the total new bone quantity and the interface of bone, also prolongs the period of new bone increasing. 2. Sr-HAP has a good biodegradation and suitable speed of biodegradating, so the reconstruction effect with Sr-HAP is fairly satisfactory.