单纯疱疹病毒Ⅰ型在兔脑神经细胞中的形态与形态发生

研究单纯疱疹病毒Ⅰ(HSV-Ⅰ)在兔脑神经细胞(RNC)中的形态与形态发生。方法 用电子显微镜观察超薄切片，并拍照记录。结果RNC感染HSV-Ⅰ6h后，细胞核中即可见散在核衣壳，12h后细胞核和细胞质内均可见到，但以核内多见，24h后病毒量达高峰。胶质细胞内的核衣壳多于神经元，胶质细胞和神经元的细胞质内和胞外可见少量有包膜的完整病毒。病毒颗粒为圆形或椭圆形，核心直径38～54nm，核衣壳77～100hm，成熟病毒115～129nm。结论RNC对HSV-Ⅰ高度敏感，HSV-Ⅰ在兔脑神经元和胶质细胞中的形态发生有一定的差异。

MORPHOLOGY AND MORPHOGENESIS OF HERPES SIMPLEX VIRUS-I IN RABBIT BRAIN NERVE CELLS

To investigate the morphology and morphogenesis of herpes simplex virus-I in rabbit brain nerve cell(RNC). Methods: Ultrathin section electron microscopy was used for study. Results:Nuleocapsids were seen in nucleus at 6h post infection,both in nucleus and cytoplasm at 12h post infection,and nearly every cell at 24h post infection. Gliacyte was more sensitive than neuron. A small number of enevloped virions were observed in nucleus and cytoplasm of gliacyte and neuron. The diameters of cores, nucleocapsids and enueloped virions are 38～54nm,77～100nm and 115～129nm respectively. Conclusion: RNC was highly sensitive to HSV-I. There was some difference in morphogenesis of HSV-I in neuron and gliacyte.