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| 骨髓间充质干细胞对缺血再灌注损伤肾小管上皮细胞Bcl-2/Bax表达的影响 | |
| 引用本文： | 刘宏宝,陈威,张鹏,赵峰,王汉民,于艳,张丰. 骨髓间充质干细胞对缺血再灌注损伤肾小管上皮细胞Bcl-2/Bax表达的影响[J]. 中国现代医学杂志, 2008, 18(17): 2437-2440 |
| 作者姓名： | 刘宏宝 陈威 张鹏 赵峰 王汉民 于艳 张丰 |
| 作者单位： | 1. 第四军医大学第一附属医院肾脏病科,陕西,西安,710032 2. 第四军医大学第一附属医院呼吸内科,陕西,西安,710032 3. 第四军医大学第一附属医院病理科,陕西,西安,710032 |
| 基金项目： | 陕西省科技攻关基金项目[No:2006K13-G5(5)] |
| 摘 要： | 目的 探讨外源性骨髓间充质干细胞(MSCs)移植对缺血再灌注损伤(I/R)后肾小管上皮细胞凋亡和Bcl-2/Bax表达的影响.方法 将雄性SD大鼠MSCs用DAPI标记后注入受体雌性SD大鼠体内.30只受体大鼠随机分为3组(n=10):假手术对照组(C组)、MSCs+I/R组(M组)、DMEM-F12+I/R组(D组).7d后观察肾功能,肾脏病理改变,采用原位末端标记法检测细胞凋亡指数,免疫组化法检测Bcl-2和Bax的表达,并观察DAPI标记的MSCs在受体大鼠肾脏的分布情况.结果 M组在肾功能、肾脏病理改变上,均明显好于D组;M组Bcl-2蛋白表达高于D组,Bax/Bcl-2比值和凋亡指数均低于D组.I/R后7 d内未发现MSCs定位于肾组织中.结论 外源性MSCs可以减少I/R损伤后肾小管上皮细胞的凋亡,促进Bcl-2表达,从而有利于肾小管损伤的早期恢复. |
| 关 键 词： | 缺血再灌注损伤 肾 间质干细胞 凋亡 基因 |
Effects of bone marrow mesenchymal stem cells on the expression of Bcl-2 and Bax proteins in renal tubular cells during ischemia-reperfusion injury in rat |
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| LIU Hong-bao , CHEN Wei , ZHANG Peng , ZHAO Feng , WANG Han-min , YU Yan , ZHANG Feng. Effects of bone marrow mesenchymal stem cells on the expression of Bcl-2 and Bax proteins in renal tubular cells during ischemia-reperfusion injury in rat[J]. China Journal of Modern Medicine, 2008, 18(17): 2437-2440 | |
| Authors: | LIU Hong-bao CHEN Wei ZHANG Peng ZHAO Feng WANG Han-min YU Yan ZHANG Feng |
| Abstract: | [Objective] To study the protective effects of bone marrow mesenchymal stem cells (MSCs) on apoptosis and the expression of Bcl-2 and Bax proteins in renal tubular cells during ischemia-reperfusion (I/R) injury in rat. [Methods] MSCs were isolated from a male SD rat and were labeled with DAPI before injection. 30 female recipient SD rats were divided randomly into three groups (n=10): control group (Group C), MSCs injection group(Group M) and DMEM-F12 infusion group (Group D). Seven days later, the serum le... |
| Keywords: | ischemia-reperfusion injury kidney mesenchymal stem cells apoptosis gene |
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