应用基因芯片技术筛选卵巢癌紫杉醇耐药差异表达基因

目的应用基因芯片技术研究人卵巢癌紫杉醇耐药细胞株OC3/Tax300与其亲本细胞OC3(敏感株)之间的基因表达谱差异,筛选耐药相关基因,探讨基因表达谱差异与卵巢癌肿瘤耐药之间相关性。方法分别提取C3/Tax300与OC3细胞的总RNA并纯化mRNA;将等量的mRNA逆转录,以Cy5和Cy3标记的cDNA做探针,在Biostar H140S基因表达谱芯片上进行杂交。扫描芯片荧光信号图像,用基因图像分析软件对扫描图像进行数字化处理和分析。结果共筛选出显著表达差异基因134条,其中117种基因表达水平下调,17种基因表达上调,主要涉及细胞信号蛋白和传递蛋白,蛋白翻译合成类以及细胞骨架和运动、离子通道和运输蛋白、代谢、发育等14大类相关基因。下调基因主要为EBNA-3、COP9、StIP1,上调的基因主要是JAK2、HSPS、NADH等。结论这些基因表达差异与卵巢癌化疗耐药有关。

Screening Differential Expression of Taxol-resistance Related Gene of Ovarian Carcinnoma by cDNA Microarray

Objective To study the gene differential expression of taxol resistance cell line OC3/Tax300 of ovarian carcinnoma compared with other parent cell OC3, screen drug-resistance related gene by cDNA microarray, and discuss the reiationship between gene expression difference and drug-resistance. Methods The cDNA retro - transcribed from equal quantity mRNA derived from OC3/Tax300 and OC3 which were labeled with Cy5 and Cy3 fluorescence as probe respectively. The mixed probe were hybridized with BiostarH140S gene expression chip. The acquired image was analyzed by software. Results 134 signitificantly differently expressed genes were screened out, of which up - and down regulated genes were 17 and 117 respectively. They were related to 14 kinds of genes. The down - regulated genes were EBNA - 3,COP9, StlPl ,and so on. The up- regulated genes were JAK2, HSPS,NADH, and so on. Conclusion These genes related to the mechnisms of drug- resistance of ovarian cancer.