川芎嗪对大鼠肠缺血再灌注损伤的作用及机制

目的 :观察川芎嗪对大鼠小肠缺血再灌注损伤的作用及其作用机制。方法 :采用大鼠常温小肠缺血 60m in—再灌注 (I/R)模型 ,治疗组动物于再灌注前 2 0 min尾静脉给予川芎嗪 8mg/kg,再灌注后立即再次注入同等剂量的川芎嗪。观察动物血压 ,肺、肠含水量 ,肺、肠 EB含量及再灌注 10 0 min死亡率等指标。结果 :1再灌后 80m in和 10 0 min时 ,I/R组血压趋于 0 ,而治疗组血压仍近于正常水平 ,与 I/R组比较 P<0 .0 1。 2 I/R组肺、肠水肿明显 ,治疗组肺、肠水肿明显减轻 ,与 I/R组比较 P<0 .0 1。 3 I/R组肺、肠毛细血管通透性升高 ,治疗组肺、肠毛细血管通透性降低 ,与 I/R组比较 P<0 .0 5。 4再灌注 10 0 min后 ,I/R组死亡率为 10 0 % ,而治疗组死亡率为 0 ,两组比较 P<0 .0 5。结论 :川芎嗪可明显减轻大鼠小肠缺血 /再灌注损伤 ,这与川芎嗪的钙拮抗作用、自由基清除作用以及抗氧化作用有关。

Protective effect and mech anism of tetramethylpyrazine on small intestinal ischemia reperfusion injury in rats

Objective:To study the effective prevention and therapy method for small intestinal ischemia/reperfusion(I/R)injury in rats Methods:Thirty SD rats were divided into three groups randomly Anesthetized SD rats underwent superior mesenteric artery occlusion for 60 min(n=10)or sham operation(n=10)and were observed for 100 min of reperfusion The rats in the other group(n=10) underwent I/R in the same way,but 20 min before reperfusion,were injected with tetramethylpyrazine(TMP)(8 mg/kg)iv TMP were given the second time following reperfusion with the same does All the rats were injected with Evans Blue(EB,15 mg/kg)50 min before sacrifice Blood pressure,water content and lung were observed to evaluate the effect of drugs and EB content of intestine Results:TMP was improved sharply(P<0 01) The tissue contained less water(P<0 01)and EB (P<0 05) The accumulating survial rate within 100 min was improved obviously(100% vs 0,P<0 05) Conclusion:TMP can attenuate small intestinal I/R injury apparently