| 广东紫珠超临界提取物的GC-MS成分分析及体外抗菌活性 |
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| 引用本文： | 贾安,杨义芳,孔德云,朱学桂. 广东紫珠超临界提取物的GC-MS成分分析及体外抗菌活性[J]. 中国医药工业杂志, 2012, 43(3): 178-181 |
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| 作者姓名： | 贾安 杨义芳 孔德云 朱学桂 |
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| 作者单位： | 1. 中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040
2. 江西中医学院 |
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| 基金项目： | 国家“重大新药创制”科技重大专项 |
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| 摘 要： | 采用超临界CO2萃取法提取广东紫珠低极性成分,应用气相色谱-质谱法分析提取物的化学组成,结合计算机检索技术初步鉴定所分离的化合物,应用色谱峰面积归一化法计算各成分的相对百分含量,并考察各分离釜提取物对金黄色葡萄球菌、大肠杆菌和白色念珠菌的体外抗菌活性。结果从超临界CO2流体萃取分离釜Ⅰ提取物中初步鉴定了27个化合物,占总离子流图峰面积的81.12%;超临界CO2流体萃取分离釜Ⅱ提取物初步鉴定了20个化合物,占总离子流图峰面积的74.60%。广东紫珠超临界CO2萃取分离釜Ⅰ、Ⅱ提取物及二者1︰1混合物对金黄色葡萄球菌、大肠杆菌和白色念珠菌显示出抗菌活性。
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| 关 键 词： | 广东紫珠 超临界CO2萃取法 气相色谱-质谱 抗菌活性 |
Component Analysis of SFE-CO2 Extracts of Callicarpa kwangtungensis Chun. by GC-MS and Antimicrobial Activity |
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| JIA An , YANG Yifang , KONG Deyun , ZHU Xuegui. Component Analysis of SFE-CO2 Extracts of Callicarpa kwangtungensis Chun. by GC-MS and Antimicrobial Activity[J]. Chinese Journal of Pharmaceuticals, 2012, 43(3): 178-181 |
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| Authors: | JIA An YANG Yifang KONG Deyun ZHU Xuegui |
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| Affiliation: | (State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry,Shanghai 200040) |
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| Abstract: | The chemical constituents of SFE-CO2 extracts of Callicarpa kwangtungensis Chun.were separated and analyzed by GC-MS.Their relative percentages were calculated with peak area normalization method.Twenty-seven compounds were identified in the SFE-CO2 extracts I accounting for 81.12% of the peak area of the total ion-current chromatogram.Twenty compounds were identified in the SFE-CO2 extracts II accounting for 74.60% of the peak area of the total ion-current chromatogram.The main constituents of SFE-CO2 extracts I,extracts II and their mixture(1︰1) had some activities against Staphylococcus aureus,Escherichia coli and Candida albicans. |
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| Keywords: | Callicarpa kwangtungensis Chun. SFE-CO2 GC-MS antibacterial activity |
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