静磁场对人牙龈成纤维细胞碱性磷酸酶活性的影响

目的:研究不同磁路设计的磁性附着体所产生静磁场对人牙龈成纤维细胞酶学的相关作用.方法:使用自主设计的磁场加载系统,产生不同强度的静磁场,对体外培养的人牙龈成纤维细胞进行不同时间的磁场加载.通过与对照组的比较,探讨磁场对该类细胞碱性磷酸酶活性的影响.结果:改变加载强度(120 mT、10 mT、0mT)或加载时间(1、3、5个加载周期),静磁场对人牙龈成纤维细胞碱性磷酸酶活性均无显著性影响(P＞0.05).结论:静磁场对牙龈成纤维细胞碱性磷酸酶活性没有影响,初步提示开放及闭合磁路系统磁性附着体所产生静磁场,对牙龈成纤维细胞的相关酶不存在生物学效应.

Effects of static magnetic field on the activity of alkaline phosphatase of human gingival fibroblasts

Objective:To investigate the effects of static magnetic fields which are generated by the open-circuit and closed-circuit dental magnetic attachments on the enzymology of human gingival fibroblasts.Method:In this study,a magnet loading system developed by our department of prosthetic dentistry was used to impose the static magnetic force on the cultured human gingival fibroblasts in vitro.The activity of alkaline phosphatase in those fibroblasts of the test and control groups was measured and compared.Result:No significant difference(P>0.05)of alkaline phosphatase P activity was found between all the groups when they were loaded with different intensity of magnetic fields or for the different period of time.Conclusion:As far as the conditions in this study are concerned,the static magnetic fields of the open-circuit magnetic attachments have no biological effects on the alkaline phosphatase of human gingival fibroblasts as well as the close-circuit ones.