| 桂皮醛对发热大鼠下丘脑蛋白质组双向凝胶电泳分析 |
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| 引用本文： | 姜楠,霍海如,李兰芳,周军,李沧海,郭淑英,姜廷良.桂皮醛对发热大鼠下丘脑蛋白质组双向凝胶电泳分析[J].中药药理与临床,2003,19(6):11-13. |
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| 作者姓名： | 姜楠 霍海如 李兰芳 周军 李沧海 郭淑英 姜廷良 |
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| 作者单位： | 中国中医研究院中药研究所唐氏中药研究中心,北京,100700 |
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| 基金项目： | 国家自然科学基金重大研究计划,项目资助号90209006 |
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| 摘 要： | 目的：观察桂皮醛对酵母致热大鼠的解热作用及其时下丘脑蛋白质组的影响。方法：采用双向凝胶电泳技术和计算机辅助图像分析方法，对蛋白质进行分离，以模型组作为参考胶进行匹配。结果：口服给予桂皮醛后时发热大鼠有解热作用，双向电泳图像分析显示桂皮醛组与模型组匹配率为86％，多个蛋白质点表达量有不同程度的改变。结论：桂皮醛时酵母致热大鼠有解热作用，其差异表达的蛋白质点可能参与了解热过程。
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| 关 键 词： | 桂皮醛 发热 下丘脑蛋白质组 双向凝胶电泳 |
Changes in hypothalamus proteins levels in febrile rat after administration of cinnamic aldehyde |
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| Jiang Nan,Huo Hairu,Li Lanfang,Zhou Jun,Li Canghai,Guo Shuying,Jiang Tingliang.Changes in hypothalamus proteins levels in febrile rat after administration of cinnamic aldehyde[J].Pharmacology and Clinics of Chinese Materia Medica,2003,19(6):11-13. |
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| Authors: | Jiang Nan Huo Hairu Li Lanfang Zhou Jun Li Canghai Guo Shuying Jiang Tingliang |
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| Abstract: | Objective: To observe the differentoial expressed protein spots in hypothalamus in yeast induced febrile rats and the yeast induced febrile rats treated with cinnamic aldehyde. Methods: Using two-dimensional polyacrylamide gel electrophoresis (2-DE) and computer-assisted image analysis to separate and find the spots of interests. The febrile group gel was designated as reference gel. Results: Cinnamic aldehyde has antipyretic effect on febrile rats. The matching rate of the control group is 91%, the matching rate of the Tested Group is 86%. A few protein spots were changed more than 30 folds. Conclusion: The spots of interests may be identified as the proteins associate with the febrile process, or the potential targets of cinnamic aldehyde. |
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| Keywords: | cinnamic aldehyde proteome two-dimensional polyacrylamide gel electrophoresis (2-DE) febrile |
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