| 高效液相色谱法测定丹参中丹酚酸B |
| |
| 引用本文： | 张启伟,张颖,李计萍,马华. 高效液相色谱法测定丹参中丹酚酸B[J]. 中国中药杂志, 2001, 26(12): 848-849 |
| |
| 作者姓名： | 张启伟 张颖 李计萍 马华 |
| |
| 作者单位： | 中国中医研究院中药研究所, |
| |
| 基金项目： | 国家重点科技项目专题(96-903-01-02) |
| |
| 摘 要： | 目的 :建立丹参中丹酚酸B高效液相色谱测定方法。方法 :75%甲醇回流提取 ,色谱柱YWGC18,流动相甲醇 5%乙酸 (35∶65 ) ,检测波长281nm。结果 :丹酚酸B0.17～1.70μg线性关系良好 (r =0.9997) ,回收率98.9% ,RSD1.82%。结论 :为丹参及其制剂的质量控制提供了分析方法。
|
| 关 键 词： | 丹参 丹酚酸B 含量测定 高效液相色谱法 |
| 文章编号： | 1001-5302(2001)12-0848-02 |
| 收稿时间： | 2000-07-03 |
| 修稿时间： | 2000-07-03 |
Determination of Salvianolic Acid B in the Radix of Salvia miltiorrhiza Bge. by HPLC |
| |
| ZHANG Qi wei;ZHANG Ying;LI Ji ping;MA Hua. Determination of Salvianolic Acid B in the Radix of Salvia miltiorrhiza Bge. by HPLC[J]. China Journal of Chinese Materia Medica, 2001, 26(12): 848-849 |
| |
| Authors: | ZHANG Qi wei ZHANG Ying LI Ji ping MA Hua |
| |
| Affiliation: | Institute of Chinese Materia Medica, China Academy of TCM, Beijing 100700, China. |
| |
| Abstract: | OBJECTIVE: To develop an HPLC method for determining salvianolic acid B in radix of Salvia miltiorrhiza. METHOD: The sample was extracted with 75% methanol. ODS column was used with methanol-5% acetic acid solution (35:65) as mobile phase. Detection wavelength was 281 nm. RESULT: Salvianolic acid B was separated well. Good linearity of salvianolic acid B was obtained (r = 0.9997) within the range of 0.17-1.7 micrograms. The average recovery was 98.9%. Repeatability was good, and RSD was 1.82%. CONCLUSION: This method can be used for quality control of radix of Salvia miltiorrhiza. |
| |
| Keywords: | radix of Salvia miltiorrhiza salvianolic acid B quantitative determination HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
