血管紧张素-2对内皮细胞和线粒体膜电位影响

目的 观察血管紧张-2(Ang-2)对血管内皮细胞(VEC)内[Ca²⁺]i及线粒体膜电位水平的影响。方法 将VEC分为空白对照组和Ang-2处理组。用激光共聚焦显微镜测量各组细胞的[Ca²⁺]i和线粒体膜电位。结果 空白对照组的细胞内[Ca²⁺]i为(24.781±1.350),Ang-2组的细胞内[Ca²⁺]i为(47.384±1.323),Ang-2组的细胞内[Ca²⁺]i,明显高于空白对照组(P<0.01)。空白对照组的线粒体膜电位水平为(36.578±1.1470),Ang-2组的线粒体膜电位水平为(9.016±1.124),Ang-2组的线粒体膜电位水平明显低于空白对照组(P<0.01)。结论 Ang-2可引起VEC内[Ca²⁺]i和线粒体膜电位水平的明显改变。

Effects of Ang-2 on intracellular [Ca2+] i and mitochondria membrane potential In VEC

Objective To explore the effects of angiotensin-2 (Ang-2) on intracellular free calcium concentration and mitochondria membrane potential in vascular endothelial cell(VEC).Methods VEC were divided into control group and Ang-2 group.Intracellular free calcium concentration and mitochondria memborne potential were measured by laser scanning confocalm icroscopy.Results Intracellular free calcium concentration of control group was 24.781±1.350.In tracellular free calcium concentration of Ang-2 group was 47.384±1.323.Intracellular free calcium concentration of Ang-2 group was significantly higher than that of control group (P<0.01).Mitochondria membrane potential of control group was 36.578.14Z Mitochondria membrane potential of Ang-2 group was 9.016±1.124 and significantly lower than that of control group (P<0.01).Conclusion Ang-2 can induce obvious changes of intracellular free calcium concentration and mitochondria memborne potential of VEC.