不同黄连炮制品HPLC指纹图谱研究

目的:建立黄连不同炮制品的HPLC指纹图谱,为黄连不同炮制品的鉴别及炮制作用机制的初探提供依据。方法:采用HPLC测定了酒制黄连等6种不同黄连炮制品样品,运用样品聚类与指纹图谱相似度评价方法分析其结果。结果:黄连不同炮制品的HPLC指纹图谱共有峰特征明显,不同炮制品指纹图谱有差异。样品聚类将黄连不同炮制品分为2类,聚类结果与药性相关联。结论:本法简便、快捷、重复性好,可用于黄连不同炮制品的鉴别。黄连不同炮制品药性的变化与炮制辅料有关,其机制可能是辅料作用产生了新的成分及使已有成分的含量发生了变化。

Fingerprints of different samples of processed Rhizoma Coptidi by HPLC

Objective: To set up the HPLC fingerprints of different samples of processed Rhizoma Coptidi(RC) in order to offer evidence for identifying different processed RC and preliminary discussion on processing mechanism.Method: Seven different samples of processed RC were determined by HPLC and the results were analyzed by Hierarchical clustering and similarity evaluation system for chromatographic fingerprint of TCM.Result: The characteristic of common peaks for fingerprints of different samples of processed RC is distinct,but there are differences among various processed RC.The different samples of processed RC were classified into two and the result of hierarchical clustering is correlated with the property of traditional Chinese drug.Conclusion: It is proved that the method which is convenient,rapid and repeat well can be used to identify different samples of processed RC.The change of property of different processed RC is related with processing adjuvant,the possible mechanism is that new components are produced or the contents of known components have changed due to the adjuvant during processing.