羊膜移植联合碱性成纤维细胞生长因子滴眼液治疗兔角膜碱烧伤

目的 观察羊膜移植与碱性成纤维细胞生长因子(bFGF)滴眼液联合应用对兔角膜碱烧伤的治疗效果。方法 将60只新西兰白兔随机分为4组,分别为羊膜移植联合bFGF滴眼液点眼组(A组),羊膜移植组(B组),bFGF滴眼液点眼组(C组)及对照组(D组)。用1 mol/L NaOH烧伤兔右眼,术后2 ～28 d用裂隙灯显微镜观察角膜透明度,新生血管生长,角膜上皮修复及羊膜植片的变化。结果 所有烧伤角膜都能完全愈合,但在抑制炎症反应、抗新生血管的生长及角膜上皮修复等方面,羊膜移植联合bFGF滴眼液点眼组明显优于对照组,也好于其他两实验组,差异有统计学意义(P＜0.05)。结论 羊膜移植联合bFGF滴眼液点眼对治疗角膜碱烧伤有良好效果。

Therapeutic effect of amniotic membrane transplantation with basic fibroblast growth factor on alkali-burned cornea in rabbit

Objective To study the effect of amniotic membrane transplantation with basic fibroblast growth factor (bFGF) on corneal alkali bum in rabbit. Methods Sixty New Zealand rabbits were divided into 4 groups randomly including amniotic membrane transplantation with bFGF ( A), amniotic membrane transplantation ( B), basic fibroblast growth factor (bFGF) (C) and negative control group (D).Each rabbit's right eye was burnt by 1 mol/L NaOH, We observe the corneal-opacity, corneal neovascularization, and the repair of corneal epithelium every day during days 2-28 after operation. Results All of corneal were cured, and group A has better curative effect than other groups in inflammatory reaction, tissue repair and anti-CNV (P ＜ 0. 05 ). Conclusion Amniotic membrane transplantation with bFGF has better curative effect for coreal alkali burn.