Galectin-3在CD133+肺腺癌细胞中表达增高诱导CD8+T细胞凋亡

目的 检测肺腺癌CD133+细胞中Galectin-3的表达.方法 磁珠分选出10例患者肺腺癌中CD133+细胞.流式细胞术检测分选效率,荧光实时定量聚合酶链反应( fqRT-PCR)和Western blot检测CD133+细胞中Galectin-3的表达,并在体外检测了CD133+细胞上清诱导CD8+T细胞凋亡的能力.结果 流式细胞术结果表明磁珠分选出的细胞中CD133+细胞数为90％.fqRT-PCR 和Western blot结果发现Galectin-3在CD133+细胞中的表达量分别为CD133-细胞中的1.24倍和1.5倍,差异有统计学意义(P＜0.05).凋亡检测结果显示CD133+细胞的上清诱导CD8+T细胞凋亡率为(27.1±2.6)％,并且这种诱导凋亡的能力可被乳糖以及抗Galectin-3的抗体中和.结论 Galectin-3在肺腺癌CD133+细胞中高表达,并具有很强的生物学活性,在体外具有明显的诱导CD8+T细胞凋亡的功能.

Expression of Galectin-3 in CD133 + pulmonary adenocarcinoma cells induced CD8 + T cell apoptosis

Objective To investigate the expression and function of Galectin-3 in CD133 + pulmonary adenocarcinoma cells.Methods CD133 + cells were separated by magnetic activated cell sorting (MACS) from resected pulmonary adenocarcinoma specimens of 10 patiens.The proportion of CD133 +cells was measured by flow cytometry (FCM).The expression of Galectin-3 in CD133 + or CD133- cells was quantitated by fluorescent quantitation real time-polymerase chain reaction (fqRT-PCR) and Western blotting.We also investigated whether the supemants of CD133 + cells could mediate apoptosis of CD8 + T cells in vitro.Results 90％ cells separated by MACS were positive for CD133,which was proved by FCM.The expression of Galectin-3 was 1.24 folds and 1.5 folds higher in CD133 + cells than that in CD133 - cells detected by fqRT-PCR and Western blotting.The supernants of CD133 + cells more effectively induced apoptosis of CD8+ T cells with apoptosis rate being (27.1 ± 2.6) ％ than those of CD133 - cells with apoptosis rate being ( 10.1 ±2.2)％,which could be down-regulated by lactose and anti-CD133 monoclonal antibody.Conclusion Galectin-3 was highly expressed in CD133 + pulmonary adenocarcinoma cells,exerted strong biological activity,and could obviously mediate CD8 + T cell apoptosis in vitro.