sPLA2在重症急性胰腺炎的变化及银杏苦内酯B对其影响

目的：探讨分泌型磷脂酶A：（sPLA2）在重症急性胰腺炎（SAP）的动态变化及银杏苦内酯B（BN52021）对sPLA2的影响。方法：180只Wistar大鼠随机分为阴性对照组（NC组，60只）、SAP模型组（SAP组，60只）、BN52021治疗组（BN组，60只）。每组按不同时相点（1、2．3、6、12和24h）分组每小组各10只，分别测定血淀粉酶和sPLA2活性水平，同时对胰腺组织进行病理学观察。结果：淀粉酶和病理学结果显示SAP制模成功，BN52021能降低SAP的血淀粉酶并改善其病理变化；sPLA2结果显示，SAP组在3、6、12和24h较NC组升高，差异有统计学意义（P＜0．05），BN组在1、2、3、6和12h较NC组升高（P＜0．05），BN组在1h较SAP组显著升高（P＜0．05），其余各个时相点差异均无统计学意义（P＞0．05）。结论：sPLA2在SAP中后期显著升高，对SAP的病情发展有重要意义，BN52021可降低SAP血淀粉酶并改善其病理变化，但对sPLA2无显著影响。

Effects of Ginkgolide B on Secretory Phospholipase A2 in Rats with Severe Acute Pancreatitis

Objective: To investigate dynamic changes of secretory phospholipase A2 (sPLA2) in rats with severe acute pancreatitis (SAP) and the effect of ginkgolide B(BN52021) on SAP. Methods: Wistar rats were randomly divided into 3 groups including negative control group (NC), SAP model group(SAP) and BN52021 treating group (BN). The rats of each group were further randomly divided into 6 subgroups at different time points including 1 h, 2 h, 3 h, 6 h, 12 h and 24 h. The activities of serous amylase and sPLA2 were detected in each subgroup, and the pathologic changes of pancreas were observed using HE stains. Results: The results of detection of serous amylase and pathology showed that SAP models were successful. The levels of sPLA2 showed significant higher in SAP group than those of NC group at 3 h, 6 h,12 h and 24 h, in BN group than those of NC group at 1 h, 2 h, 3 h, 6 h and 12 h and in BN group than that of SAP group at 1 h (P < 0.05 for each). Conclusion: The sPLA2 increased remarkably in metaphase and anaphase of SAP and played an important role in SAP. BN52021 could reduce serous amylase and ameliorate pathology of pancreas, but had no effect on the activities of sPLA2.