不同年龄人肝解偶联蛋白2表达与衰老的关系

目的:探讨解偶联蛋白2(UCP_2)在人肝组织中的表达及其与衰老的关系。方法:选取临床人肝组织,按年龄分为胎儿组、成年组和老年组,分别取各组肝组织测定丙二醛(MDA)、谷胱甘肽(GSH)、三磷酸腺苷(ATP)的含量;通过免疫组织化学法观察肝组织中UCP_2的表达与分布。结果:MDA含量老年组高于胎儿组和成年组;GSH含量老年组低于胎儿组和成年组;ATP水平老年组低于胎儿组和成年组;UCP_2在老年组肝组织实质细胞内表达较多,在胎儿组、成年组肝组织实质细胞内表达较少,甚至没有表达。结论:老年人肝组织MDA含量升高,GSH含量降低,提示老年人肝组织内活性氧水平较高,并引起UCP_2表达增多,UCP_2在肝细胞的损伤与衰老过程中起重要作用。

Relationship between expression of uncoupling protein 2 and aging in human liver at different ages

Objective : To explore the expression of uncoupling protein 2(UCP2) in human liver tissues and its relationship with aging. Methods :Clinical human liver tissues were selected and divided into fetal group,adult group and elderly group according to ages. The content of malondialdehyde(MDA) and glutathione(GSH), adenosine triphosphate(ATP) were determined from each group. The expression and distribution of UCP2 were studied in liver tissues by immunohistochemistry. Results : MDA of the elderly group was higher than that of the fetal group and the adult group. The GSH content in the elderly group was lower than that in the fetal group and the adult group. The ATP level was lower in the elderly group than that in the fetal group and the adult group. UCP2 protein was expressed more in the liver cells of the elderly group, and was less expressed(or even not expressed) in the liver cells of the fetal group and the adult group. Conclusion : The MDA content increases and the GSH content decreases in the liver tissue of the elderly,suggesting the increased level of reactive oxygen species(ROS) in the liver tissue of the elderly results in the increased expression of UCP2. UCP2 plays an important role in the process of the damage and aging in liver cells.