BioOcularTM角膜上皮模型ET50法在化妆品体外眼刺激评价中的应用

目的 采用国内生产的BioOcular?三维人源重组角膜上皮模型,对市面常见的几种洗发水和面霜的眼刺激性进行比较,通过该方法评价不同配方的化妆品在眼刺激性上的强弱。方法 采用三维人源重组角膜上皮模型BioOcular?模型 ET50法对不同品牌洗发水和面霜的眼刺激性进行比较研究,区分不同配方产品的眼刺激性强弱等级。结果 在同样实验条件下,洗发水和面霜表现出对三维人源重组角膜不同的刺激程度,不同配方品牌的洗发水的刺激程度也有很大差别。该方法可对化妆品的眼刺激性强弱程度进行很好的区分。结论 采用三维人源重组角膜上皮模型的ET50法是一种评价不同配方化妆品以及原料的眼刺激性的差异的快速替代检测方案。

Evaluation of Eye Irritation Potential of Cosmetics Using Human Cornea-like Epithelial Tissue Model BioOcularTM ET50 Method in Vitro

ABSTRACT: OBJECTIVE This study aims to evaluate eye irritation potential of different shampoos and facial cream using reconstructed human cornea-like epithelial tissue model in vitro. METHODS Different shampoos and facial creams were tested in eye irritation ET50 test with reconstructed human cornea-like epithelial tissue model BioOcular? in vitro. RESULTS Under the same condition, shampoos and facial creams showed irritation at various levels in ET50 test with reconstructed cornea-like epithelial tissue model. Also different ingredients and different brand of shampoos show different degrees of irritation. Thus, it is a good method to evaluate eye irritation potential of cosmetics with reconstructed human corneal epithelium model. CONCLUSION It is a quick alternative way to use ET50 method to evaluate the irritation of different cosmetics and ingredients with reconstructed human cornea-like epithelial tissue model BioOcular? in vitro.