复方卡那霉素眼药水中卡那霉素和地塞米松的含量测定

目的：解决复方卡那霉素眼药水中卡那霉素和地塞米松的含量测定问题。方法：在复方卡那霉素眼药水中加入衍生试剂,使卡那霉素形成二氢吡啶衍生物,利用其在紫外有吸收的特点进行测定。地塞米松的测定因受到对羟基苯甲酸乙酯的干扰,所以采用双波长分光光度法进行测定。结果：卡那霉素在８～２０μｍ／ｍｌ范围内呈线性ｒ＝０．９９９８,地塞米松在１２～２０μｇ／ｍｌ范围内呈线性ｒ＝０．９９９８,卡那霉素和地塞米松的平均回收

Determination of kanamycin and dexamethasone in the compound kanamycin eye drops

OBJECTIVETo determine theconcentrations of kanamycin and dexamethasone in the eye-drops.METHODKanamycinwas derivatized in eye-drops to form dihydropyridine derivative,and the concentrati ons ofdihydropyridine derivative was determined by UV.Dual-wavelength spectrop hotometry wasused to determine the dexamethasone.RESULTSThe linear ranges of thedetermination of kanamycin and dexamethasone were 8～20μg/ml(r=0.9998) and 12～20μg/ml(r=0.9998),respectively.The avera ge recovery ofkanamycin was 99.92% with RSD 0.40% and 99.52% with RSD 0.42%,respectively.CONCLUSIONThemethod is rapid,sim ple and accurate for the assay of kanamycin and dexamethasone.