支气管哮喘患儿MPO、PMN及MCP-4的血清水平及临床意义

目的 探讨外周血清过氧化物酶(myeloperoxidase，MPO)、中性粒细胞(neutrophils，PMN)及单核细胞趋化蛋白-4(monocyte chemoattractant protein-4，MCP-4)在小儿支气管哮喘的血清水平，为临床有效防治提供有效参考。方法 采用酶联免疫吸附法分别测定67例支气管哮喘患儿(哮喘组)和30例健康儿童(正常对照组)血清MPO、PMN及MCP-4水平，并进行比较。结果 1)MPO的血清水平在支气管哮喘急性发作期为(878.43±18.77)U/L] 高于哮喘缓解组(678.62±33.36)U/L]及健康对照组(703.17±18.26)U/L](P均＜0.05);2)MCP-4在支气管哮喘急性发作期患儿血清水平(117.34±18.77)pg/mL]高于哮喘缓解组(55.67±6.13)pg/mL]及健康对照组(33.74±2.98)pg/mL](P均＜0.05)。3)PMN绝对值在支气管哮喘急性发作期(10.31±1.98)×10⁹/L]高于哮喘缓解组(4.63±1.51)×10⁹/L]及健康对照组(4.82±1.51)×10⁹/L](P均＜0.05)。MPO、PMN及MCP-4的升高程度与病情严重程度相关，且两者之间呈正相关(r=0.976，0.989;P均<0.01)。结论 MPO、PMN及MCP-4在支气管哮喘中发挥了重要作用，与气道炎症反应有关，血清MPO、PMN及MCP-4可做为支气管哮喘的生化检测指标。哮喘缓解期仍存在慢性炎症反应。

Serum levels and clinical significance of myeloperoxidase,neutrophils and monocyte chemoattractant protein-4 in children with asthma

Objective To investigate the levels of myeloperoxidase(MPO),neutrophils (PMN) and monocyte chemoattractant protein-4 (MCP-4) in serum of children with bronchial asthma,and to explore the clinical significance. Method The serum levels of MPO,PMN and MCP-4 in 67 cases of bronchial asthma children (asthma group) and 30 healthy children (control group) were measured and compared by using enzyme-linked immunosorbent assay (ELSA). Results 1) The serum level of MPO (878.43±18.77) U/L ]in acute exacerbation of bronchial asthma was higher than that in the remission group (678.62±33.36) U/L ]and healthy controls (703.17±18.26) U/L ](P<0.05).2)The serum level of MCP-4 in the acute attack of bronchial asthma children (117.34±18.77) pg/mL] was higher than that in the remission group (55.67±6.13pg/ml) and healthy control group (33.74±2.98) pg/mL](P<0.05).3) The absolute value of PMN in acute attack of bronchial asthma (10.31±1.98)×10⁹/L] was higher than that in the remission group (4.63±1.51)×10⁹/L] and healthy control group (4.82±1.51)×10⁹/L] (P<0.05).The elevated levels of MPO,PMN and MCP-4 were related to the severity of the disease,and there was a positive correlation between the two(r=0.976,0.989;all P<0.01). Conclusions MPO,PMN and MCP-4 play an important role in bronchial asthma,and is related to airway inflammation.Serum MPO,PMN and MCP-4 can be used as a biochemical indexes of bronchial asthma.The remission of asthma are chronic inflammatory reaction.