注意缺陷多动障碍儿童体内锌含量及T淋巴细胞活性的研究

目的 研究注意缺陷多动障碍(attention deficit hyperactivity disorder,ADHD)患儿体内锌含量及T淋巴细胞亚群活性,以促进ADHD患儿的治疗。方法 选择60例ADHD患儿和50例正常对照儿童,采用原子吸收光谱法测定血锌含量,同时采用流式细胞仪检测CD3⁺、CD4⁺、CD8⁺T淋巴细胞亚群活性水平。结果 ADHD组患儿血锌含量(73.24±9.89)μmol/L,明显低于正常对照组(82.51±7.81)μmol/L,t=5.373,P<0.01]。ADHD组患儿CD3⁺、CD4⁺、CD4⁺/CD8⁺T淋巴细胞活性(57.9±5.0)%,(26.8±3.9)%,1.059±0.234]较正常对照组(68.2±4.9)%,(36.5±4.7)%,1.432±0.279]显著降低,差异均有统计学意义(t=10.84,11.85,7.63,P<0.01),而两组CD8⁺无明显差别。相关分析表明血锌与CD4⁺、CD3⁺呈正相关(r=0.577,0.411,P<0.01)。结论 ADHD患儿血锌含量偏低,存在T淋巴细胞亚群失衡状态,以CD4⁺T淋巴细胞活性降低为主,补充锌制剂有利于ADHD患儿的治疗。

Blood zinc level and activity of T lymphocyte in children with attention deficit hyperactivity disorder

Objective To study the blood zinc level and activity of T lymphocyte in children with attention deficit hyperactivity disorder (ADHD),and to promote the therapy of ADHD. Methods Blood zinc levels of 60 ADHD patients and 50 healthy controls were detected by atomic absorption spectrometry,at the same time,the T lymphocyte subsets of CD3⁺,CD4⁺ and CD8⁺ in peripheral blood of the two groups were measured by flow cytometer. Results The blood zinc level was lower in ADHD children (73.24±9.89) μmol/L] than that in health controls (82.51±7.81) μmol/L,t=5.373,P<0.01].Levels of CD3⁺、CD4⁺、CD4⁺/CD8⁺ T lymphocyte subsets in ADHD group(57.9±5.0)%,(26.8±3.9)%,1.059±0.234],had significantly depressed,as compared with those of healthy controls(68.2±4.9)%,(36.5±4.7)%,1.432±0.279,t=10.84,11.85,7.63,P<0.01].Level of CD8⁺ had no difference between the two groups.Correlation analysis showed that the blood zinc level was positively correlated with level of CD4⁺ and CD3⁺ T lymphocyte (r=0.577,0.411,P<0.01). Conclusion The blood zinc level decreased significantly,the balance of T lymphocyte subsets was disorder,and the decrease of CD4⁺ T lymphocyte was the most evident in ADHD children.Addition of zinc can make for the therapy of ADHD.