高效液相色谱法测定人脑脊液中利奈唑胺浓度

目的:建立高效液相色谱法快速测定人脑脊液中利奈唑胺浓度并对危重患者进行药物浓度监测。方法:采用外标法,色谱柱:ZORBAX Edipse XDB-C18(4.6 mm×150 mm,5μm);流动相:乙腈-水(23∶77);流速:1.0mL·min-1;柱温:30℃;紫外检测波长:254 nm,并利用建立的方法对危重患者进行利奈唑胺脑脊液浓度的监测。结果:利奈唑胺在0.31～40 mg.L-1范围内线性关系良好(r=0.999 9),最低定量限为0.31 mg.L-1,平均绝对回收率为46.8%,平均相对回收率为96.99%,高、中、低3个浓度日内、日间精密度的RSD均小于<5%。另外,所监测的危重患者中,利奈唑胺不同时期在脑脊液中的浓度变化很大。结论:所建立的高效液相色谱法简单、快捷、灵敏、准确,可用于监测利奈唑胺在脑脊液中的浓度。

Determination of linezolid concentration in human cerebrospinal fluid by high performance liquid chromatography

OBJECTIVE To establish a method for determining the concentration of linezolid in human cerebrospinal fluid by HPLC,and to determine linezolid concentration in craniocerebral injury patients.METHODS The external standard method was used,the separation was performed on a ZORBAX Edipse XDB-C18(4.6 mm×150 mm,5 μm),the mobile phase consisted of acetonitrile:water(23∶77) with a flow rate of 1.0 mL·min-1,the UV detection wavelength was 254 nm.RESULTS The calibration curves of linezolid showed good linear regression in the range of 0.31-40 mg·L-1(r=0.999 9),the limits of detection was 0.31 mg·L-1.The mean absolute recovery was 46.8%,and the relative recovery was 96.99%.Intra and inter-day variations were all less than 5%.In addition,in craniocerebral injury patients,linezolid concentration in cerebrospinal fluid varied much in the different periods of disease.CONCLUSION The established method is simple,rapid,sensitive and accurate for determining linezolid level in human cerebrospinal fluid.