| Pirh2 shRNA增强肺腺癌细胞A549对顺铂敏感性的研究 |
| |
| 引用本文： | 苏远,朱莉萍,金阳,张晓菊,周琼,白明. Pirh2 shRNA增强肺腺癌细胞A549对顺铂敏感性的研究[J]. 华中科技大学学报(医学版), 2008, 37(2): 170-174. DOI: 10.3870/j.issn.1672-0741.2008.02.008 |
| |
| 作者姓名： | 苏远 朱莉萍 金阳 张晓菊 周琼 白明 |
| |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院呼吸科,武汉,430022
2. 华中科技大学同济医学院基础医学院卫生部呼吸系疾病重点实验室,武汉,430030 |
| |
| 摘 要： | 目的 观察短发夹状RNA沉默泛素连接酶pirh2(P53 induced RING-H2 protein)基因表达后,肺腺癌细胞A549对顺铂敏感性及肺耐药蛋白(lung resistance-related protein, LRP)表达的变化.方法 构建靶向pirh2基因的短发夹状RNA(psiRNA-Pirh2)及阴性对照RNA(psiRNA-Con),并转染A549细胞,实时定量PCR和免疫印迹法检测A549细胞中pirh2基因和蛋白的表达变化.设立顺铂组(5 μg/ml)、psiRNA-Pirh2转染组和顺铂(5 μg/ml) psiRNA-Pirh2组,CCK-8法检测A549细胞增殖能力,流式细胞术检测细胞周期,免疫印迹法检测肺耐药蛋白的表达,以正常A549细胞为对照.结果 psiRNA-Pirh2特异性抑制了A549细胞pirh2 mRNA和蛋白质的表达(均P＜0.05).顺铂及psiRNA-Pirh2组A549细胞增殖能力分别为正常对照组的(46.82± 6.14)%和(37.45±6.83)%,均P＜0.05,但均高于二者联合组[(28.24±6.49)%],且联合组细胞凋亡率最高,达(19.8±5.1)%,S期细胞最少,为(15.5±1.6)%(P＜0.05).顺铂处理组A549细胞LRP表达较正常对照组增强(P＜0.05),但联用psiRNA-Pirh2后,LRP蛋白水平有所下降(P＜0.05).结论 靶向pirh2的shRNA可特异性降低A549细胞中pirh2的表达,抑制A549细胞增殖,使其阻滞在G2/M期,诱导凋亡,下调肺耐药蛋白的表达并增强其对顺铂的化疗敏感性.
|
| 关 键 词： | RNA干涉 Pirh2 泛素 肺肿瘤 顺铂 shRNA 增强 肺腺癌细胞 顺铂敏感性 研究 Cisplatin Cell Line Lung Chemosensitivity 化疗敏感性 诱导凋亡 阻滞 细胞增殖 蛋白水平 处理 细胞凋亡率 正常对照组 蛋白质 mRNA 结果 |
| 修稿时间： | 2006-12-30 |
Effect of Pirh2 shRNA on Chemosensitivity of Lung Adencarcinoma Cell Line A549 to Cisplatin |
| |
| Su Yuan,Zhu Liping,Jin Yang et al. Effect of Pirh2 shRNA on Chemosensitivity of Lung Adencarcinoma Cell Line A549 to Cisplatin[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2008, 37(2): 170-174. DOI: 10.3870/j.issn.1672-0741.2008.02.008 |
| |
| Authors: | Su Yuan Zhu Liping Jin Yang et al |
| |
| Affiliation: | Su Yuan1,Zhu Liping2,Jin Yang1 et al 1Department of Respiratory Diseases,Union Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 4300222Department of Pathophysiology,School of Basic Medical Sciences,Pulmonary Disease Laboratory of Ministry of Health of China,Wuhan 430030 |
| |
| Abstract: | Objective To investigate the effects of pirh2 short hairpin RNA(shRNA) on the pirh2 expression,cell proliferation,apoptosis and chemosensitivity of lung adencarcinoma cell line A549 to cisplatin.Methods Two shRNA plasmids were constructed,one of which was designed targeting pirh2(named psiRNA-Pirh2),and the other targeting nothing as negative-control(named psiRNA-Con).Both of them were transfected into A549 cells by LipofectamineTM 2000 respectively.Real-time PCR and Western-blotting were applied to detect ... |
| |
| Keywords: | RNA interference Pirh2 ubiquitin lung neoplasm cisplatin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
