| 白英药材的HPLC指纹图谱 |
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| 引用本文： | 甄会贤,刘文娟,程候莲,齐 伟,陈 杨,孙立新.白英药材的HPLC指纹图谱[J].沈阳药科大学学报,2009,26(4):285-289. |
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| 作者姓名： | 甄会贤 刘文娟 程候莲 齐 伟 陈 杨 孙立新 |
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| 作者单位： | 甄会贤,ZHEN Hui-xian(沈阳药科大学,药学院,辽宁,沈阳,110016;山西生物应用职业技术学院,山西,太原,030031);刘文娟,程候莲,LIU Wen-juan,CHENG Hou-lian(山西生物应用职业技术学院,山西,太原,030031);齐伟,陈杨,孙立新,QI Wei,CHEN Yang,SUN Li-xin(沈阳药科大学,药学院,辽宁,沈阳,110016)
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| 基金项目： | 沈阳市科技局资助项目,辽宁省科技厅项目 |
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| 摘 要： | 目的研究白英药材的HPLC指纹图谱。方法采用RP-HPLC法,色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为乙腈-质量分数为0.2%的甲酸溶液二元梯度洗脱模式,流速为1.0 mL.min-1,检测波长为270 nm,建立21批白英样品的HPLC指纹图谱。结果对各产地药材指纹图谱进行聚类分析,根据聚类分析结果将21批白英分为4类,选定其中12批样品建立共有模式。并应用"中药指纹图谱相似度评价系统"对各产地药材进行了质量评价。结论该法可用于白英真伪鉴别和质量评价。
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| 关 键 词： | 白英 黄酮 有机酸 高效液相指纹图谱 |
HPLC fingerprint of whole plant of Solanum lyratum Thunb. |
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| ZHEN Hui-xian,LIU Wen-juan,CHENG Hou-lian,QI Wei,CHEN Yang,SUN Li-xin.HPLC fingerprint of whole plant of Solanum lyratum Thunb.[J].Journal of Shenyang Pharmaceutical University,2009,26(4):285-289. |
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| Authors: | ZHEN Hui-xian LIU Wen-juan CHENG Hou-lian QI Wei CHEN Yang SUN Li-xin |
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| Institution: | 1.School of pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2.Shanxi Biological Application Vocational Technical College, Taiyuan 030031, China |
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| Abstract: | Objective To study HPLC fingerprint of Solanum lyratum Thunb. Methods RP-HPLC fingerprint analysis method was developed. Kromasil C18 column(250 mm×4.6 mm,5 μm）was used with mixture of acetonitrile and acid water as mobile phase in a gradient mode. The flow rate was 1.0 mL·min-1 .The wavelength was 270 nm.Twenty-one batches of Solanum lyratum. Thunb.were determined. Results Twenty one samples were classified as four clusters by cluster analysis and the mutual model from 12 batches of sample were established. The samples’ quality was assessed by Similarity Evaluation System for chromatographic fingerprint of TCM 2004. Conclusion The method can be used conveniently to identify and evaluate the quality of Solanum lyratum Thunb.. |
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| Keywords: | Solanum lyratum Thunb flavonoids organic acids HPLC fingerprint |
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