脑脉舒康胶囊对短暂性脑缺血模型小鼠的影响

目的:探讨脑脉舒康胶囊对短暂性脑缺血发作小鼠模型的影响。方法:采用昆明种小鼠,分为脑脉舒康胶囊组(1.8,0.9,0.45 g.kg-1)、模型组、假手术和阳性药(血栓通1.125 g.kg-1、尼莫地平0.03 g.kg-1)对照组,各组连续灌胃7 d,于第8天给药1 h后,结扎两侧颈总动脉30 min,并进行再灌注15 min制备短暂性脑缺血模型;眼球取血,测定超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量;取部分小鼠脑组织匀浆,检测脑匀浆液Na+-K+-三磷酸腺苷酶(ATPase),Mg2+-ATPase,Ca2+Mg2+-ATP,Ca2+-ATPase活力;部分脑组织称重后计算脑含水量。结果:脑脉舒康胶囊可以增强Na+-K+-ATPase,Mg2+-ATPase,Ca2+-Mg2+-ATP,Ca2+-ATPase活力,降低MDA含量,提高SOD的活性,减轻脑缺血引起的脑组织间质水肿。结论:脑脉舒康胶囊可提高脑组织ATP能量,稳定离子泵功能,清除氧自由基,缓解脑水肿,具有保护脑细胞的作用。

Experimental Study of Naomai Shukang Capsule on Transient Ischemic Attack in Mice

Objective:To study the effects of Naomai Shukang capsule on transient ischemic attack in mice. Method: Kunming mice were divided into Naomai Shukang capsule group (1.8,0.9,0.45 g·kg^-1), the model group, sham operation control group,and the positive control group (Xueshuantong 1.125 g·kg^-1,nimodipine 0.03 g·kg^-1), mice were fed Naomai Shukang capsule for 7 d, on the 8 d, after 1 h of administration, both sides of the common carotid artery were ligatured for 30 min and reperfusion for 15 min to make transien cerebral ischemia model; superoxide dismutase(SOD)and malonaldehyde(MDA) content were determinated; section of brain tissue was homogenated, Na⁺-K⁺-adenosine triphosphatase(ATPase), Mg²⁺-ATPase, Ca²⁺-Mg²⁺-ATP,Ca²⁺-ATPase activity of brain homogenate were measured, part of brain tissue was calculated after weighing for brain water content. Result: Naomai Shukang capsule could enhance Na⁺-K⁺-ATPase, Mg²⁺-ATPase, Ca²⁺-Mg²⁺-ATP,Ca²⁺-ATPase activity in brain,and decrease MDA content, increase SOD activity, brain edema was relieve induced by cerebral ischemia. Conclusion: Naomai Shukang capsule can improve ATP energy of brain, scaveng oxygen free radicals, relieve cerebral edema, protect brain cells, reduce brain structure and function damage.