蛋白质组学及其研究技术在肺癌分子生物标志物研究中的应用

肺癌是全球发病率和死亡率较高的恶性肿瘤。细胞从正常状态转变为肿瘤的过程中，细胞内蛋白质表达谱必然会发生一系列变化，肿瘤分子标记物就是细胞在非正常状态下产生的分子．以高通量结合生物信息学为特点的蛋白质组学分析技术可以从细胞整体水平上检测到这种变化近年来，为了降低肺癌的死亡率并提高其治疗效果，研究人员利用这一新的手段如利用激光捕获显微切割技术和双相凝胶电泳结合基质辅助激光解析电离飞行时间质谱（MALDI-TOF MS）和表面增强激光解析电离色行时间质谱（SELDI—TOF MS）寻找有效的肺癌标记物。本文对蛋白质组学技术在肺癌生物标志物研究中的应用作了综述。

Application of proteomic techniques in searching lung cancer biomarkers

Lung cancer is one of the malignant tumors with a high morbitity and mortality in the worht. During its neoplastic development, a set of changes in protein expression occurs. The changed proteomie profile of cancers-tumor biomarkers could guide clinical decision-making. Proteonlic methods are widely used in identification of tumor bionlarkers.To reduce tile mortality of lung eaneer and improve its therapy, nlany studies on the identification of this tumor biomarker are being carried out. This review summarizes the main strategies and technology in proteomics and its application in identification of lung cancer bionlarkers.