| 液相色谱-串联质谱法测定原发性肝癌患者血清中7种甾类激素 |
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| 引用本文： | 钱跹,吕磊,战旗,李悦悦,李云青,张国庆,赵亮. 液相色谱-串联质谱法测定原发性肝癌患者血清中7种甾类激素[J]. 第二军医大学学报, 2016, 37(7): 810-814. DOI: 10.16781/j.0258-879x.2016.07.0810 |
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| 作者姓名： | 钱跹 吕磊 战旗 李悦悦 李云青 张国庆 赵亮 |
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| 作者单位： | 第二军医大学东方肝胆外科医院药材科,上海,200438 |
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| 摘 要： | 目的 建立高效液相色谱-串联质谱(LC-MS/MS)法,用于测定原发性肝癌患者血清中7种甾类激素的含量.方法 血清样品采用乙酸乙酯液-液萃取,经丹磺酰氯衍生化后测定.色谱柱为Agilent Poroshell 120 EC-C18柱(2.1 mm×150mm,2.7μ.m),流动相为乙腈-0.1％(V∶V)甲酸水溶液,梯度洗脱,流速为0.3 mL/min,柱温为35℃,质谱检测采用动态多反应离子监测模式(MRM).结果 7种甾类激素(雌二醇、雌三醇、雌酮、氢化可的松、睾酮、雄烯二酮和黄体酮)在8 min内达到基线分离.在相应浓度范围内线性关系良好,相关系数均＞0.99,日内及日间精密度RSD均＜15％,提取回收率为80％～119％,基质效应为85％～112％,样品在放置6h后处理、处理后室温放置24 h、3个冻融循环以及-80℃保存30 d等测定过程中均稳定性良好.结论 该方法操作简便、结果准确、灵敏度高、重现性好,适用于原发性肝癌患者体内甾类激素水平检测.
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| 关 键 词： | 液相色谱-串联质谱 甾类激素 肝肿瘤 肝细胞癌 |
| 收稿时间： | 2016-02-19 |
| 修稿时间： | 2016-03-19 |
LC-MS/MS for determination of seven steroid hormones in sera of primary hepatocellular carcinoma patients |
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| QIAN Xian,L,#;Lei,ZHAN Qi,LI Yue-yue,LI Yun-qing,ZHANG Guo-qing and ZHAO Liang. LC-MS/MS for determination of seven steroid hormones in sera of primary hepatocellular carcinoma patients[J]. Former Academic Journal of Second Military Medical University, 2016, 37(7): 810-814. DOI: 10.16781/j.0258-879x.2016.07.0810 |
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| Authors: | QIAN Xian,L&# Lei,ZHAN Qi,LI Yue-yue,LI Yun-qing,ZHANG Guo-qing ZHAO Liang |
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| Affiliation: | Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University,Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Second Military Medical University |
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| Abstract: | |
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| Keywords: | LC-MS/MS steroid hormone primary liver cancer determination |
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