鳖甲煎口服液对大鼠肝纤维化的影响

目的 :研究鳖甲煎口服液抗肝纤维化的作用机理。方法 :用四氯化碳复合因素造模 ,给予鳖甲煎口服液治疗。观察血清透明质酸 (HA)、层粘连蛋白 (LN )的变化 ,部分标本观察肝细胞超微结构的变化。结果 :鳖甲煎口服液能降低血清HA、LN含量 ,减少肝细胞变性、坏死和纤维增生程度 ,减轻超微结构的病理变化。结论 :鳖甲煎口服液对实验性肝纤维化有一定的治疗作用 ,其机理可能与保护肝细胞功能 ,抑制星状细胞合成和分泌过量细胞外基质 ,减轻肝血窦毛细血管化 ,改善肝脏微循环 ,调节机体免疫功能有关。

Therapeutic research of Biejia Jian oral liquid (鳖甲煎口服液,BOL) in hepatofibrotic rats

Objective: To study the anti hepatofibrosis mechanism of BOL. Methods: The model was induced by subcutaneous injection of CCl 4, BOL was administered and the change of serum hyaluronic acid (HA) and laminin (LN) was observed and the degeneration of liver cells and the degree of fibre hyperplasia analyzed. Changes of ultra micro structure in liver cells were observed in some samples. Results: HA was reduced in both the groups with low and high dosage of BOL, which showed a remarkable difference as compared with that of the model group. The LN content of low and high dosage group of BOL was lower than that of model group and colchicine group. Examined by Ridit, BOL could reduce the degeneration and necrosis of liver cells (X 2=11.99, P<0.05), the degree of fibre hyperplasia (x 2=13.24, P<0.05) and the pathological change of ultra micro structure as well. Conclusion: The BOL has certain therapeutic effect on the experiment hepatofibrosis. Its mechanism might be: protecting the function of liver cells, inhibiting excessive synthesis and secretion of extracelluar matrix from hepatic stellate cells, relieving the capillarization of hepatic sinusoid, improving liver microcirculation and regulating immune function.