MSP法检测胃癌组织中RASSF1A基因启动子区甲基化改变

目的：探讨抑癌基因RASSF1A启动子区CpG岛甲基化与胃癌及临床病理特征的关系。方法：采用甲基化特异性PCR（methylation—specific PCR，MSP）法检测60例胃癌组织及相应癌旁组织和30例对照组织中RASSF1A基因启动子区甲基化状态。结果：胃癌组织中RASSF1A基因启动子区CpG岛甲基化率为65．0％（39／60），艋著高于癌旁组织6．7％（4／60），及对照组0％（0／30）（P〈0．01）。胃癌组织中不同年龄、性别、分化程度及淋巴结转移与否的RASSF1A基因甲基化率的差异均无统计学意义。结论：胃癌中RASSF1A基因启动子区的高甲基化提示其与胃癌的发生密切相关，MSP法对RASSF1A基因启动子区甲基化的检测有望成为胃癌早期监测的重要方法。

Detection of promoter methylation of RASSF1A gene in gastric cancer by MSP

Objective：To detect promoter methylation of RASSFI A gene in gastric cancer and to analyze their relationship with the clinical and pathological features. Methods： Methylation - specific polymerase chain reaction （MSP） was used to detect promoter methylation of RASSF1A gene in gastric cancer tissues and their adjacent tissues from 60 patients, and in 30 normal specimens. Results：The positivity rate of promoter methylation of RASSFI A gene was significantly higher in gastric cancer than that in the corresponding non - neoplastic tissues and normal controls （65.0% vs 6.7% and 0% ,P 〈0.01 ）. No correlation was found between promoter methylation status of RASSF1A gene and age, gender, tumor differentiation, and lymph node metastasis in patients with gastric cancer. Conclusion： Hypermethylation of RASSF1A gene is present in the gastric cancer tissues, suggesting the hypermethylation of RASSF1A might be associated with the tumorigenesis of gastric cancer. It may provide help in the early detection of gastric cancer.