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| survivin反义寡核苷酸在体外诱导胰癌细胞凋亡 | |
| 引用本文： | 熊平源,郭凯文,唐朝晖. survivin反义寡核苷酸在体外诱导胰癌细胞凋亡[J]. 中国免疫学杂志, 2005, 21(11): 832-834 |
| 作者姓名： | 熊平源 郭凯文 唐朝晖 |
| 作者单位： | 1. 武汉科技大学医学院免疫学教研室,武汉,430080 2. 华中科技大学同济医学院,武汉,430000 |
| 基金项目： | 本课题为湖北省卫生厅科技基金资助项目（JX1B039） |
| 摘 要： | 目的：探讨survivin反义核酸对胰腺癌细胞株Panc-1细胞凋亡的影响.方法：用脂质体瞬时转染法介导survivin反义核苷酸处理胰腺癌Panc-1细胞后,MTT试验测定转染细胞的相对存活率,RT-PCR检测survivin mRNA表达,琼脂糖凝胶电泳分析其对Panc-1细胞的凋亡诱导作用.结果：转染survivin反义核酸的Panc-1细胞增殖明显受抑制,与对照进行比较,具有显著性差异（P＜0.05）;经琼脂糖凝胶电泳,转染survivin反义核酸的Panc-1细胞可见到DNA梯形条带,而对照细胞未见到.结论：survivin反义核酸能够诱导Panc-1细胞凋亡. |
| 关 键 词： | 反义RNA survivin 胰腺癌 凋亡 |
| 文章编号： | 1000-484X（2005）11-0832-03 |
| 收稿时间： | 2005-01-29 |
| 修稿时间： | 2005-01-292005-04-28 |
Apoptosis of pancreatice cancer cells induced by antisense RNA of survivin gene in vitro |
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| XIONG Ping-Yuan,GUO Kai-Wen,TANG Zhao-Hui. Apoptosis of pancreatice cancer cells induced by antisense RNA of survivin gene in vitro[J]. Chinese Journal of Immunology, 2005, 21(11): 832-834 | |
| Authors: | XIONG Ping-Yuan GUO Kai-Wen TANG Zhao-Hui |
| Affiliation: | Department of Immunology, College of Medical Science, Wuhan University of Science and Technology, Wuhan 430080, China |
| Abstract: | Objective:To explore the effects of survivin antisense RNA on apoptosis in pancreatic cancer cell Panc-1.Methods:survivin antisense RNA was transferred into pancreatic cancer cell Panc-1 by lipofection.The opposite livability on Panc-1 cell line were assayed with MTT test.Expression of survivin detected by RT-PCR.Apoptosis was detected by DNA gel electrophoresis.Results:survivin antisense RNA obviously inhibited the Panc-1 cell growth(P<0.05).Agarose gel electrophoresis of genomic DNA from survivin antisense RNA transferred Panc-1 showed typical DNA ladder,but DNA from other cells did not.Conclusion:survivin antisense RNA can effectively induce apoptosis in Panc-1 cells. |
| Keywords: | Anfisense RNA survivin Pancreatic cancer Apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |