草乌甲素对Balb/c小鼠的部分免疫功能的抑制作用

目的:研究草乌甲素对Balb/c小鼠部分免疫功能的影响。方法:Balb/c小鼠随机分为空白对照组、草乌甲素3个剂量(0.08、0.16、0.32 mg·kg~(-1),肌注7d)组、氢化可的松组(阳性对照药,10mg·kg~(-1))。小鼠d7处死,检测药物对小鼠胸腺指数、脾脏指数的影响;测定小鼠T、B淋巴细胞转化功能;酶联免疫法(ELISA)测定小鼠血清总IgG水平;中性红比色法测定小鼠腹腔巨噬细胞吞噬功能;硝酸还原酶法测定巨噬细胞培养上清一氧化氮(NO)水平。结果:草乌甲素0.32 mg·kg~(-1)能抑制致裂原刺激T、B淋巴细胞增殖(P<0.01),降低腹腔巨噬细胞培养上清中NO水平(P<0.05)。草乌甲素(0.16、0.32 mg·kg~(-1))降低小鼠的胸腺指数,降低小鼠血清中总IgG水平(P<0.05,P<0.01),草乌甲素还能抑制腹腔巨噬细胞吞噬功能(P<0.05,P<0.01)。结论:草乌甲素对Balb/c小鼠的免疫功能有一定的抑制作用。

Inhibitory effect of bulleyaconitine A on some immune function in Balb/c mice

AIM:To determine the effect of hulleyaconitine A(BLA)on some immune functions of mice.METHODS:Balb/c mice were divided randomly into control group,BLA groups(0.08,0.16,0.32 mg·kg~(-1),intramuscularly),and hydrocortisone(10 mg·kg~(-1))group,and drugs were given for 7d.After mice were sacrificed on d7,spleen-and thymus-index were recorded,and lymphocytes proliferation were tested.The levelof total IgG in serum was measured by ELISA method.Phagocytosis of peritoneal macrophages(MΦ)was tested with neutral-red phagocytosis assay.Nitric oxide(NO)in supernatants of macrophages was detected by nitrate reductase method.RESULTS:BLA 0.32 mg·kg~(-1)inhibited T-and B-lymphocytes proliferations(P<0.01) and NO in supernatants(P<0.05).BLA 0.16 and 0.32 mg·kg~(-1)lowered the thymus-index(P<0.05,P<0.01) with the suppression of phagocytosis function of MΦ(P<0.05,P<0.01).BLA also reduced the total IgG in serum(P<0.05,P<0.01).CONCLUSION:BLA had the suppressive effect on some immune function of Balb/c mice.