3种经典工艺生产的金银花提取物中糖类成分含量比较

目的比较3种经典工艺生产的金银花提取物中果糖、葡萄糖、蔗糖和总糖的含量差异，为指导含金银花中成药的生产和质量控制提供科学依据。方法用经典的水-醇法、石-石虎法和改良石-硫法生产金银花提取物样品，用高效液相-梯度洗脱法测定3种提取物样品中果糖、葡萄糖和蔗糖含量，用分光光度法测定3种提取物样品中总糖含量。结果3种提取物中果糖含量分别为5．44％、3．47％、4．74％，葡萄糖含量分别为4．35％、2．73％、4．21％，蔗糖含量分别为17．41％、8．47％、14．47％，总糖含量分别为50．63％、41．12％、35．90％。结论测定了金银花提取物中除绿原酸类以外的36％～51％总成分含量，并揭示了其中19％～27％的化学成分组成为果糖、葡萄糖、蔗糖。

Determination of Saccharide in Honeysuckle Extracts Produced by Three Classical Methods

Objective To determine the contents of fructose, glucose, sucrose and total saccharide in honeysuckle extracts which were produced by three classical methods, to provide scientific data for producing and controlling of those patent medicines containing honeysuckle extract. Methods Three classical extract methods were used for producing honeysuckle extracts. HPLC-ELSD method was used for determining the contents of fructose, glucose, sucrose, and a spectrophotometric determination method was used for determining the contents of total saccharide. Results The contents of fructose in three honeysuckle extracts were 5.44%, 3.47%, 4.74%, glucose were 4.35%, 2.73%, 4.21%, glucose were 17.41%, 8.47%, 14.47%, and total saccharide were 50.63%, 41.12%, 35.90%, respectively. Conclusion About 36%-51% of total components of honeysuckle extracts were determined except chlorogenic acids, and 19%-27% of components in the extracts were fructose, glucose, sucrose.