银杏叶提取物对兔外周血干细胞动员的影响

目的:探讨银杏叶提取物对兔外周血干细胞的动员作用。方法:30只26²8周龄新西兰大白兔,体重为2.528周龄新西兰大白兔,体重为2.5³.0kg,随机分三组:银杏叶提取物组(Egb)、G-CSF组、生理盐水组,给药方法为腹腔注射7d,1次/d;给药后23.0kg,随机分三组:银杏叶提取物组(Egb)、G-CSF组、生理盐水组,给药方法为腹腔注射7d,1次/d;给药后2⁸d,采用外周血WBC和MNC计数、造血干细胞体外培养、免疫细胞化学等检测各组外周血WBC、MNC、CD34+细胞、CD49d阳性细胞、CFU-GM、CFU-MK、CFU-E的产率及兔骨髓基质细胞中VCAM-1阳性细胞百分率。结果:Egb组给药第7天外周血WBC、MNC数量达到高峰,分别为给药前的3倍和3.5倍;Egb组CD49d阳性细胞、外周血CD34+及骨髓基质细胞的VCAM-1阳性细胞百分率分别为(9.37±3.01)%、(0.63±0.37)%和(48.13±9.84)%,均高于生理盐水组;其中CFU-GM、CFU-MK和CFU-E产率分别为(12.94±3.03)%、(11.49±2.93)%和(24.52±8.62)%,均高于生理盐水组。结论:银杏叶提取物(Egb)对兔骨髓造血细胞有一定的增生作用,同时对外周血干细胞有一定的动员作用,这可能与其上调兔骨髓基质细胞和外周血粘附分子的表达有关。

The effects of peripheral blood stem cell mobilization on rabbits with ginkgo biloba extract

Objective ：To study Ginkgo biloba extract （Egb） for mobilizing peripheral blood hematopoiet-ic stem cells and the effects of Egb on the expression of adherent molecule of peripheral blood hematopoietic stem cells and bone marrow cells in rats .Methods ：Thirty rabbits were divided into three groups at random （n= 10 each）：rabbits were intraperitoneally injected with Group Egb and rhG-CSF （Group G-CSF） respectively from 1 day to 7 day ,normal control with equivalence saline .The effects of the numbers of MNC and WBC the percentage of CD34＋ cell and CD49d positive cell and the yields of CFU-GM、CFU-MK、CFU-E in peripheral and the percentage of VCAM-1 positive cells in bone marrow stromal cells of the mice were observed by means of WBC and MNC counts , flow cytometry ,hematopoietic cell culture in vitro and immunocytochemistry .Results ：7 days after be treated of Egb group ,the numbers of WBC and MNC reached the maximum ,which were 3 and 3 .5 times that of the NS group re-spectively ;the perce-ntage of CD34＋ cells and CD49d positive cells in peripheral blood and VCAM-1 positive cells in bone marrow stromal cells in Egb group was （0 .63 ± 0 .37）% 、（9 .37 ± 3 .01）% and （48 .13 ±9 .84）% respectively , were markedly higher than in NS group ;the yields of CFU-GM 、CFU-MK and CFU-E in group of Egb was （12 .94 ± 3 .03）% 、（11 .49 ± 2 .93）% and （24 .52 ± 8 .62）% respectively ,were markedly higher than those in NS group . Conclusion ：Egb could mobilize hematopoietic stem cells into peripheral blood ,which role may be related to increase adhesion molecule expression in peripheral blood cells and bone marrow stromal cell in rabbits .