化痰散结方含药血清诱导人肺癌细胞凋亡的机理研究

为探讨中药化痰散结方诱导人肺癌细胞 (SPC A1)凋亡的作用及其对正常人胚肺细胞的毒性作用 ,通过血清药理学方法 ,选择不同浓度的含药兔血清与人肺癌细胞共同孵育处理 ,并以正常人胚肺细胞 (HEL)作对照。结果 :人肺癌细胞经含中药兔血清作用 ,由贴壁生长而逐渐脱落 ,外形变圆 ,体积缩小 ,折光性增强 ;荧光镜下可见细胞核破碎 ,裂解为大小不等的凋亡小体 ;流式细胞仪检测显现典型的细胞凋亡峰 ,以 5%含药血清作用 4 8h凋亡峰最为明显 ,凋亡率为 4 2 .8% ;出现S期细胞阻滞 ;而该含药血清对人胚肺细胞无诱导凋亡作用。化痰散结方通过血清药理学方法诱导人肺癌细胞的凋亡 ,可能是其抗肿瘤的分子学机制之一。

Research on the Mechanism of Induced Apoptosis of SPC-A1

Objective: Approach to the effect of composed Chinese drug 892 on inducing the apoptosis of SPC A1. Methods: According to the basic TCM theory, the pathologic characteristic of lung cancer is stagnation of phlegm and turbid qi . Drug 892, composed of Banxia (Rhizoma Pinelliae), Tiannanxing (Rhizoma Arisaematis), and Sanqi (Radix Notoginseng). After incubating SPC A1 and drug-bearing rabit serum which at different concentration with serologic pharmacological method, observed and detected the growth and apoptosis of cells, cell cycle and apoptosis rate. Results: By the action of drug bearing rabit serum, SPC A1 grew against the wall and then peeled off gradually; fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different size; FCM showed a typical peak of apoptosis, which was most obvious after 48 hours' action with 5% drug bearing rabit serum at the rate of 42.82%. Conclusion: One of the antineoplastic molecular mechanism of composed Chinese drug 892 may be its effect on inducing the apoptosis of SPC A1 by serologic pharmacological method.