右美托咪定对大鼠脑创伤后炎症反应及认知功能的影响

目的 观察右美托咪定对大鼠脑创伤后炎症反应及认知功能的影响。方法 将20只成年雄性SD大鼠随机分为实验对照（CON）组和右美托咪定（DEX）组，各10只。应用可控皮层撞击法制备大鼠颅脑外伤模型。DEX组大鼠清醒后即可腹腔注射右美托咪定100 μg/kg，对照组不予处理，两组大鼠分别在创伤后6 h、24 h、72 h采用ELISA法对其血清TNF-α、IL-6及IL-1β水平进行检测，并进行新物体识别测试。结果 两组血清TNF-α、IL-6、IL-1β水平在脑创伤6 h、24 h、72 h比较，差异有统计学意义（P＜0.05），两组TNF-α、IL-6水平在24 h下降，并于72 h再次升高，且CON组高于DEX组，差异有统计学意义（P＜0.05）；两组IL-1β水平在24 h、72 h时升高，且CON组高于DEX组，差异有统计学意义（P＜0.05）；各个时间点两组大鼠新物体识别测试结果比较，差异无统计学意义（P＞0.05）。结论 右美托咪定可减轻大鼠脑创伤后炎症反应，是否影响恢复期认知功能还需进一步观察。

Effects of Dexmedetomidine on Post-traumatic Inflammatory Response and Cognitive Function in Rats

Abstract:Objective To observe the effects of dexmedetomidine on inflammatory reaction and cognitive function in rats after traumatic brain injury.Methods 20 adult male Sprague-Dawley rats were randomly divided into experimental control(CON)group and dexmedetomidine(DEX)group,10 each.A rat model of traumatic brain injury was prepared by controlled cortical impingement.The rats in group DEX could be intraperitoneally injected with dexmedetomidine 100μg/kg,and the control group was not treated.The two groups of rats were detected the serum levels of TNF-α,IL-6 and IL-1β in the serum of 6 h,24 h and 72 h after the trauma,and the new object recognition test was carried out.Results The levels of serum TNF-α,IL-6 and IL-1β in the two groups were compared at 6 h,24 h and 72 h after brain injury,the difference was statistically significant(P<0.05).The levels of TNF-α and IL-6 in the two groups decreased at 24 h and increased again at 72 h,and the CON group was higher than the DEX group,the difference was statistically significant(P<0.05);the IL-1β level in the two groups was 24 h and 72 h were elevated,and the CON group was higher than the DEX group,the difference was statistically significant(P<0.05).There was no significant difference in the new object recognition test results between the two groups at each time point(P>0.05).Conclusion Dexmedetomidine can alleviate the post-traumatic inflammatory reaction in rats,and it is necessary to further observe whether it affects the cognitive function during recovery.