单个核细胞促血浆凝固活性监测急性排斥反应的实验研究

目的：探讨将外周血单个核细胞的促血浆凝固活性用于急性排斥反应监测的可能性。方法：将新西兰白兔随机分为３组：第１组做处体皮肤移植,第２,３组做异体皮肤移植,第３组于术前１ｄ至术后７ｄ皮下注射环孢霉素Ａ５ｍｇ（ｋｇ．ｄ）。术后每天观察衔以片的变化。

Study on monitoring acute graft rejection by PCA of MNC

Objective: The sequential changes of procoagulant activity(PCA) level in mononuclear cells (MNC) of rabbits which received skin allograft were studied in the paper in order to assess it as a marker for monitoring of acute graft rejection (AGR). Methods: New Zealand white rabbits(NZW)were random divided into three groups: rabbits in group 1(control) received autogenous skin transplants; rabbits in group 2, 3 received allogenic skin transplants(a grey rabbit as a donor), and rabbits in group 3 were treated with cyclosporin A (CsA) at 5 mg/(kgd) once daily subcutaneously for up to 9 days since the day before operation. Heparinized peripheral blood was drawn from each animal on the day of the operation and every other day after the operation. MNC were separated by FicollHypaque before PCA measurement. The activated lymphocytes were counted at the same time. Morphologic changes of the skin grafts were observed every day after transplant operation. Results: PBMNC of the animals which received allograft had larger amounts of PCA before the day of AGR, in addition, donor antigen during culture of PBMNC further enhanced PCA(DAgPCA) level in animals with AGR. Conclusion: PCA may be as markers of AGR. DAgPCA is a specific marker.