|
|||||
|
|
| 脂多糖增强小鼠腹腔巨噬细胞14.2 kD蛋白质磷酸化 | |
| 引用本文： | 刘彦,王寅,崔肇春. 脂多糖增强小鼠腹腔巨噬细胞14.2 kD蛋白质磷酸化[J]. 中国免疫学杂志, 1999, 15(10): 452-454 |
| 作者姓名： | 刘彦 王寅 崔肇春 |
| 作者单位： | [1]大连医科大学生化教研室 [2]辽宁师范大学化学系应化教研室 |
| 基金项目： | 国家自然科学基金，大连市科委基金 |
| 摘 要： | 目的：探索ＬＰＳ对小鼠腹腔巨噬细胞小分子蛋白质磷酸化的影响。方法：利用同侠素掺入，ＳＤＳ－ＰＡＧＥ，放射自显影和蛋白质印迹，结合蛋白激酶抑制剂与激活剂的作用，研究 ＬＰＳ对巨噬细胞处理后小分子蛋白质磷酸化及其相关蛋白激酶对此磷酸化的影响。结果：１４．２ｋＤ蛋白磷酸化被ＬＰＳ强烈地促进；ＭＡＰＫ的表达量未见明显变化；ＴＰＫ抑制剂三羟异黄酮明显抑制上述磷酸化；ＰＫＡ激活剂佛斯可林和Ｇｉ蛋白抑制剂百日咳 |
| 关 键 词： | 脂多糖 巨噬细胞 蛋白质磷酸化 |
Lipopolysaccharide enhances a 14.2 kD protein phosphorylation of murine peritoneal macrophages |
|
| LIU Yan,WANG Yin,CUI Zhao Chun. Lipopolysaccharide enhances a 14.2 kD protein phosphorylation of murine peritoneal macrophages[J]. Chinese Journal of Immunology, 1999, 15(10): 452-454 | |
| Authors: | LIU Yan WANG Yin CUI Zhao Chun |
| Affiliation: | LIU Yan,WANG Yin,CUI Zhao Chun.Department of Biochemistry,Dalian Medical University,Dalian 116027 |
| Abstract: | Objective:This study is aimed at exploring the influence of LPS on the phosphorylation of small MW proteins in mouse peritoneal macrophages.Methods:Radioisotope incorporation,SDS PAGE,autoradiography,Western blot and protein kinase inhibitor and activator were used.Results:LPS strongly enhanced the phosphorylation of a 14.2 kD protein; MAPK expression was not significantly influenced by LPS;the TPK inhibitor genistein significantly inhibited this phosphorylation;the PKA activator forskolin and Gi protein inhibitor pertussis toxin did not show influence on the 14.2 kD protein phosphorylation.Conclusion:LPS enhanced the phosphorylation of a 14.2 kD protein and this phosphorylation might be TPK dependent. |
| Keywords: | Lipopolysaccharide Macrophage Protein phosphorylation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |